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AQUABLUER

               a Bluer cell viability assay solution

AquaBluerTM is a proprietary redox indicator. Viable cells turn AquaBluer from its oxidized form (nonfluorescent, blue) to the reduced form (fluorescent, red). The fluorescence intensity of AquaBluer at 540ex/590em is proportional to the number of viable cells in the sample. It can be used to assess cell viability, cell proliferation, and cytotoxicity. It is nontoxic, simple to use, sensitive, reproducible, and has a broad assay range.

 

 


 

Comparison of Alamar Blue and AquaBluer
 
 

 

 Alamar Blue

 AquaBluer

Detection Mechanism

Redox Indicator

Redox Indicator

Product Form

Homogeneous Solution

Stabilized Homogeneous Solution

Price

~$350 /100 ml

$199 /15 ml

# of Assays in 96-Well

10,000

 15,000

Linear Assay Range

600-75,000 cells/well

600-150,000 cells/well

 
alamar_aquabluer assay range2.jpg
 
 
 

 


Brief Protocol 

1. Set up a cytotoxicity assay

Seed the cells at 6000-8000 cells/100 ul/well in 96-well culture plates and let the cells grow overnight. Add serially diluted test compounds to each well. Include 4 “No-cells” and 4 “No-drug” wells as 0% and 100% viability controls. Return the plate to the CO2 incubator for 48-72 hrs.

2. Start AquaBluer assay

Add 100 ul AquaBluer solution (15 ml in the kit for 15,000 assays) to 10 ml culture medium in a dispensing reservoir. Pipette up and down to mix well. Replace the culture medium in each well with the 100X diluted AquaBluer medium. Alternatively, directly add 1 ul of undiluted AquaBluer solution to each 100 ul well, especially for viability assay of suspension cultures. Return the plate to the CO2 incubator for 4 hrs.

3. Raw data acquisition

Remove the plate from the incubator. Place the plate in a fluorescence plate reader and read the fluorescence intensity at 540ex/590em.

4. Viability and IC50 calculation

Subtract the average of fluorescence value (RFU) of the No-cell control (background) from all other RFU values. Convert the test RFU values to % viability using the formula:

% Viability = (RFUtest / RFUveh) x 100, where RFUveh is the average RFU of the No-drug wells. Enter the % viability values and corresponding log test compound concentrations into a non-linear regression program such as Prism or online calculator such as BioDataFit at http://www.changbioscience.com/stat/ec50.html to obtain the IC50 values of the test compounds.




 

  

Ordering Information

Product Name:    AquaBluerTM Kit

Product Number:   6001, 6015

Application:    Assessing cell viability, cell proliferation, and cytotoxicity.

Size:    The kit is sufficient for performing

      • 6001: 1,000 assays in 96-well format using 1 ul AquaBluer in 100 ul medium
      • 6015: 15,000 assays 

Kit Contents:  The AquaBluer Kit includes the following items        

  • 60011 ml AquaBluer solution, Instruction Manual
  • 601515 ml AquaBluer solution, Instruction Manual

Price:   

      • 6001: $10 each
      • 6015: $199 each

Ordering:    To order, please click the "BUY" button below.  

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