AquaBluerTM
is a proprietary redox indicator. It does not interact with the
cells but measures the redox environment in the medium. Viable cells
maintain a reduced environment and turn AquaBluer from
its oxidized form (nonfluorescent, blue) to the reduced form
(fluorescent, red). The fluorescence intensity of
AquaBluer
at 540ex/590em is proportional to the number of viable cells in the
sample. Therefore, it can be used to assess cell viability, cell
proliferation, and cytotoxicity. It is nontoxic, simple to use,
sensitive, reproducible, and has a broad assay
range.