AquaGenomicTM is an aqueous reagent for DNA extraction. It may be used to extract DNA from all types of specimens, from bacteria to animal tissues. The extraction protocols are simple, fast, and scalable. AquaGenomic is nontoxic and its lysate may be used for PCR without further DNA purification. AquaGenomic can efficient extract DNA from dried specimen swabs. It facilitates the use of dried specimen swabs for low-cost biobanking, biosurveillance and epidemiological studies.
AquaGenomic is a multi-functional aqueous solution for genomic DNA isolation and purification. It achieves cell lysis, DNA extraction, and debris removal in a single step.
Add AquaGenomic solution to lyse the cells, extract the DNA, and precipitate the cell debris. The crude lysate may be used directly for PCR analysis without further DNA purification.
No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no multiple solutions and buffers.
No phenol, no chloroform, no isoamylacohol, no saturated butanol, no guanidine hydrochloride.
One AquaGenomic Kit can be used to isolate DNA from cultured cells, buccal swabs, blood, bacteria, animal or plant tissues. No need to purchase different kits for different starting materials.
One single AquaGenomic Kit does all: mini, midi, or maxi. No need to purchase separate mini, midi, or maxi kits.
About 5-10 ug of DNA can be isolated from 1-2 million cultured cells, 15 mg of feces, 1 ml of bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues.
AquaGenomic is priced at $199/kit for 300 minipreps, that is $0.66/miniprep or 1/4 of the price of other genomic DNA isolation kits.
Below is a comparison of AquaGenomic and a leading competitor. You can easily see the advantages and benefits of using AquaGenomic.
Comparison of genomic DNA isolated from different mouse tissues by AquaGenomic. Approximately 10 mg of tissue or 100 ul of blood were processed using the AquaGenomic Kit. Final genomic DNA was suspended in 50 ul deionized water. Five-microliter of each prep was run in a 1% agarose gel.
(Please refer to the Instruction Manual for DNA extraction from bacteria, tissues, soil, saliva, blood, etc.)
AquaGenomic Cell Protocol
This protocol can be used to prepare 5-10 ug of genomic DNA from 1-2 million cultured cells. For other sample sizes, use additional 100 ul of AquaGenomic Solution for each million nucleated cells.
Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 12,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: For convenience, cell culture may be mixed with 1 vol of AquaGenomic for DNA extraction without needing to pellet the cells.)
To use the lysate for PCR, dilute an aliquot of the lysate with 10-20 vol of
water and then use 0.25 ul of the diluted
lysate in a 25-ul PCR reaction.)
0.8 vol of 100% isopropanol (e.g., to 100 ul crude lysate, add 80 ul
isopropanol) and vortex for 30-60 sec to mix well. Centrifuge at 12,000
xg for 5 min to pellet the DNA. Decant to discard the supernatant.
AquaGenomic Swab Protocol
Cotton swabs are commonly used in forensic evidence collection. However, existing DNA extraction methods can recover only ~100-500 ng of DNA from a dried specimen swab as the majority of DNA remains entrapped within the cotton matrix. With AquaGenomic, ~5 ug of DNA can be extracted from a dried specimen swab. It is possible to use cotton swabs for specimen collection, transportation, storage, and DNA extraction for low-cost biobanking, biosurveillance, and epidemiological studies.
1. Collect the specimen. Use a cotton swab to soak up the specimen (~100-200 ul), such as blood, saliva, mucus, semen, feces, cultured mammalian or bacteria cells, homogenized animal or plant tissues, or any other potential sources of biospecimens. Air-dry the specimen swab at ambient temperature (20-50 °C) for >24 hours. The dried specimen swabs can be shipped at ambient temperature and then stored at room temperature in sealed paper envelopes or plastic bags with desiccant for many years.
2. Extract the proteins and small molecules (dried blood swabs). Cut off the specimen swab tip into a 1.5-ml microfuge tube. Add 400 ul deionized water. Soak the swab for >30 min. Use a 1-ml pipet tip to smash the swab ~10 times and press it to the bottom of the tube to squeeze out the solution. Transfer as much liquid as possible (~300 ul) to a new 1.5-ml microfuge tube for analysis of plasma proteins or small molecules.
3. Extract the DNA. Add 300 ul AquaGenomic to the 1.5-ml tube containing the wet swab from Step 2 or a new dried specimen swab. Incubate at 75-85 °C for 30 min. Centrifuge briefly to collect any condensation. Use a 1-ml blue pipet tip to smash the swab ~10 times and press it to the bottom of the tube to squeeze out the solution. Alternatively, homogenize the samples in AquaGenomic in 0.5-ml screw capped tubes with a multichannel bead beater. Transfer as much liquid as possible (~200 ul) to a new 0.5-ml microfuge tube or use a microfuge spin bucket to recover all the lysate from the swab to maximize DNA yield (Note: To use the crude lysate for PCR, dilute an aliquot of the crude lysate with 10-20 vol of deionized water and use 0.25 ul of the diluted lysate in a 25-ul PCR reaction).
4. Purify the DNA. Add 0.8 vol of isopropanol to the crude lysate (e.g., add 160 ul to 200 ul lysate). Vortex to mix well and centrifuge at 12,000 xg for 5 min to pellet the DNA. Flip the tube to discard the isopropanol supernatant. Gently shoot 70% ethanol solution from a squirt bottle to fill up the tube. Flip the tube to discard the ethanol solution. Tap and place the tube upside down on a clean paper towel to remove residual solution. Air-dry the DNA pellet for 5-10 min. Add 100 ul of TE buffer or deionized water to the DNA pellet, pipette or vortex vigorously to suspend the DNA. Incubate at 65-75 °C for 15 min. Centrifuge at 12,000 xg for 5 min to pellet any insoluble and transfer the clear DNA solution to a new tube.
Product Name: AquaGenomicTM Kit
Product Number: 2001, 2030
Application: Isolation of genomic DNA from cells and tissues. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaGenomic Kit includes the following items
Ordering: To order, please click the "BUY" button below.
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