AquaGenomicTM is a novel multi-functional aqueous solution for
the isolation and purification of genomic DNA. This single
aqueous solution allows the completion of cell suspension, cell
lysis, DNA extraction, and debris removal in one
step. AquaGenomic
is a user and environment friendly reagent and it contains no
chemical that is harsher than SDS, a component of everyday
detergents.
AquaGenomicis a multi-functional aqueous solution
for genomic DNA isolation and purification.
It achieves cell lysis, DNA extraction, and debris removal in a
single step.
Simple
Add AquaGenomic
solution to lyse the cells, extract the DNA, and precipitate
the cell debris, and then add isopropanol to precipitate the
purified genomic DNA.
Easy
No columns, no
resins, no slurries, no beads, no filters, no membranes, no
needles, no cartridges, no manifolds, no multiple solutions and
buffers.
Nontoxic
No phenol, no
chloroform, no isoamylacohol, no saturated butanol, no guanidine
hydrochloride.
Fast
Two minutes to
lyse cells and extract DNA, four minutes to pellet cell
debris, two minutes to pellet DNA. From cells or tissues,
to DNA in ~15 minutes.
Pure
A260/A280 of the
isolated genomic DNA is 1.6-1.8. The genomic DNA
is free from enzyme inhibitors.
Flexible
One AquaGenomic Kit
can be used to isolate DNA from cultured cells, buccal swabs,
blood, bacteria, animal or plant tissues. No need to purchase
different kits for different starting materials.
Scalable
One single
AquaGenomic Kit does all: mini, midi, or maxi. No need to purchase
separate mini, midi, or maxi kits.
High
Yield
About 5-10 ug of
DNA can be isolated from from
1-2 million cultured cells, 200 ul of whole blood, 15 mg of feces, 1
ml of bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of
plant tissues.
Economic
AquaGenomic is
priced at $199/kit for 300 minipreps, that is $0.66/miniprep
or 1/4 of the price of other genomic DNA isolation
kits.
Comparison
Below is a
comparison of AquaGenomic and a leading competitor. You can easily
see the advantages and benefits of using
AquaGenomic.
Competitor Q
AquaGenomic
Isolation
Mechanism
Selective Binding
Selective Extraction
Isolation Format
Solid
Support
Aqueous
Solution
Price ($/miniprep)
2.10-6.64
0.66
Proteases
Yes
No
Chaotropic
Salt
Yes
No
Multiple Kits
Yes, mini, midi, maxi; animal, plant,
blood, swab, etc.
No, a single kit for all sizes
and materials
# of Solutions in the
Kit
6-7
1
Isolation Time
(min)
30 (mini)
60 (midi)
90 (maxi)
4-16 hours (tissue)
15 (mini)
15 (midi)
15 (maxi)
15 (tissue)
Yield (ug
DNA)
10 (mini)
60 (midi)
600
(maxi)
10
(mini)
100 (midi)
1000 (maxi)
Purity
(A260/A280)
1.7-1.9
1.6-1.8
Scalable
No
Yes (100 ul AG Solution/million
cells)
Comparison of genomic DNA isolated from different
mouse tissues by AquaGenomic. Approximately 10 mg of tissue or 100
ul of blood were processed using the AquaGenomic Kit.
Final genomic DNA was suspended in 50 ul deionized
water. Five-microliter of each prep was run in a 1%
agarose gel.
Ordering
Information
Product
Name: AquaGenomicTM Kit
Product Number:
2001, 2030
Application:
Isolation
of genomic DNA from cells and tissues. For in vitro research
use only.
Size: The
kit is sufficient for the preparation of
2001: 10
minipreps
2030: 300
minipreps, or 30 midipreps, or 3
maxipreps
Kit Contents: The
AquaGenomic Kit includes the following
items
2001: 1 ml AquaGenomic Solution,
Instruction Sheet
2030: 30 ml AquaGenomic Solution,
Instruction Manual
Price:
2001: $10 each
2030: $199 each
Ordering: To order,
please click the "BUY"
button below.
