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AQUAGENOMIC

AquaGenomicTM is an aqueous reagent for DNA extraction. It may be used to extract DNA from all types of specimens, from bacteria to animal tissues. The extraction protocols are simple, fast, and scalable. AquaGenomic is nontoxic and its lysate may be used for PCR without further DNA purification. AquaGenomic can efficient extract DNA from dried specimen swabs. It facilitates the use of dried specimen swabs for low-cost biobanking, biosurveillance and epidemiological studies.

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Features

Innovative

AquaGenomic is a multi-functional aqueous solution for genomic DNA isolation and purification. It achieves cell lysis, DNA extraction, and debris removal in a single step.

 

Simple

Add AquaGenomic solution to lyse the cells, extract the DNA, and precipitate the cell debris. The crude lysate may be used directly for PCR analysis without further DNA purification.

Easy

No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no multiple solutions and buffers.

Nontoxic

No phenol, no chloroform, no isoamylacohol, no saturated butanol, no guanidine hydrochloride.

Flexible

One AquaGenomic Kit can be used to isolate DNA from cultured cells, buccal swabs, blood, bacteria, animal or plant tissues. No need to purchase different kits for different starting materials.

Scalable

One single AquaGenomic Kit does all: mini, midi, or maxi. No need to purchase separate mini, midi, or maxi kits.

High yield

About 5-10 ug of DNA can be isolated from 1-2 million cultured cells, 15 mg of feces, 1 ml of bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues.

Economic

AquaGenomic is priced at $199/kit for 300 minipreps, that is $0.66/miniprep or 1/4 of the price of other genomic DNA isolation kits.

Comparison

Below is a comparison of AquaGenomic and a leading competitor. You can easily see the advantages and benefits of using AquaGenomic.

   Competitor Q  AquaGenomic
 Format  Solid support  Aqueous Solution
 Price ($/mini)  2.10-6.46  0.66
 Chaotropic salt  Yes  No
 Multiple kits  Yes, mini, midi, maxi; animal, plant, blood, etc.  No, one kit for all sizes and materials
 # of solutions  6-7  1
 Lysate for PCR  No  Yes
 Scalable  No  Yes

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Comparison of genomic DNA isolated from different mouse tissues by AquaGenomic. Approximately 10 mg of tissue or 100 ul of blood were processed using the AquaGenomic Kit. Final genomic DNA was suspended in 50 ul deionized water. Five-microliter of each prep was run in a 1% agarose gel. 

 


Brief Protocol


(Please refer to the
Instruction Manual for DNA extraction from bacteria, tissues, soil, saliva, blood, etc.)



AquaGenomic Cell Protocol

This protocol can be used to prepare 5-10 ug of genomic DNA from 1-2 million cultured cells. For other sample sizes, use additional 100 ul of AquaGenomic Solution for each million nucleated cells.


1. Harvest the Cells

Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 12,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: For convenience, cell culture may be mixed with 1 vol of AquaGenomic for DNA extraction without needing to pellet the cells.)

 
2. Extract the DNA

 Add 100 ul of AquaGenomic to the cell pellet. Suspend and lyse the cells by vortex vigorously for 30-60 sec. Incubate at 22 C for 5 min. (Optional: Incubate at 75 C for 20 min for best DNA yield, DNase inactivation and RNA degradation.)

(Note: To use the lysate for PCR, dilute an aliquot of the lysate with 10-20 vol of water and then use 0.25 ul of the diluted lysate in a 25-ul PCR reaction.)

 
3. Pellet the DNA

Add 0.8 vol of 100% isopropanol (e.g., to 100 ul crude lysate, add 80 ul isopropanol) and vortex for 30-60 sec to mix well. Centrifuge at 12,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant.

4. Rinse the DNA

Gently fill up the tube with 70% ethanol from a squirt bottle, and then flip the tube to discard the ethanol solution. Repeat the 70% ethanol rinse once. Tap the tube on a paper towel to remove residual ethanol solution and leave it upside down for 5-10 min to air-dry the DNA pellet.

5. Solubilize the DNA

Add 100 ul of TE buffer or deionized water to the DNA pellet, pipette or vortex vigorously to suspend the DNA. Incubate at 22 C for 15 min (Optional: To fully solubilize and recover the DNA, incubate at 65-75 C for 15 min.). Centrifuge at 12,000 xg for 5 min to pellet the insoluble and transfer the clear DNA solution to a new tube.





AquaGenomic Swab Protocol


Cotton swabs are commonly used in forensic evidence collection. However, existing DNA extraction methods can recover only ~100-500 ng of DNA from a dried specimen swab as the majority of DNA remains entrapped within the cotton matrix. With AquaGenomic, ~5 ug of DNA can be extracted from a dried specimen swab. It is possible to use cotton swabs for specimen collection, transportation, storage, and DNA extraction for low-cost biobanking, biosurveillance, and epidemiological studies.

1. Collect the specimen. Use a cotton swab to soak up the specimen (~100-200 ul), such as blood, saliva, mucus, semen, feces, cultured mammalian or bacteria cells, homogenized animal or plant tissues, or any other potential sources of biospecimens. Air-dry the specimen swab at ambient temperature (20-50 C) for >24 hours. The dried specimen swabs can be shipped at ambient temperature and then stored at room temperature in sealed paper envelopes or plastic bags with desiccant for many years.

2. Extract the proteins and small molecules (dried blood swabs). Cut off the specimen swab tip into a 1.5-ml microfuge tube. Add 400 ul deionized water. Soak the swab for >30 min. Use a 1-ml pipet tip to smash the swab ~10 times and press it to the bottom of the tube to squeeze out the solution. Transfer as much liquid as possible (~300 ul) to a new 1.5-ml microfuge tube for analysis of plasma proteins or small molecules.

3. Extract the DNA. Add 300 ul AquaGenomic to the 1.5-ml tube containing the wet swab from Step 2 or a new dried specimen swab. Incubate at 75-85 C for 30 min. Centrifuge briefly to collect any condensation. Use a 1-ml blue pipet tip to smash the swab ~10 times and press it to the bottom of the tube to squeeze out the solution. Alternatively, homogenize the samples in AquaGenomic in 0.5-ml screw capped tubes with a multichannel bead beater. Transfer as much liquid as possible (~200 ul) to a new 0.5-ml microfuge tube or use a microfuge spin bucket to recover all the lysate from the swab to maximize DNA yield (Note: To use the crude lysate for PCR, dilute an aliquot of the crude lysate with 10-20 vol of deionized water and use 0.25 ul of the diluted lysate in a 25-ul PCR reaction).

4. Purify the DNA. Add 0.8 vol of isopropanol to the crude lysate (e.g., add 160 ul to 200 ul lysate). Vortex to mix well and centrifuge at 12,000 xg for 5 min to pellet the DNA. Flip the tube to discard the isopropanol supernatant. Gently shoot 70% ethanol solution from a squirt bottle to fill up the tube. Flip the tube to discard the ethanol solution. Tap and place the tube upside down on a clean paper towel to remove residual solution. Air-dry the DNA pellet for 5-10 min. Add 100 ul of TE buffer or deionized water to the DNA pellet, pipette or vortex vigorously to suspend the DNA. Incubate at 65-75 C for 15 min. Centrifuge at 12,000 xg for 5 min to pellet any insoluble and transfer the clear DNA solution to a new tube.


 


 
Ordering Information


Product Name:     AquaGenomicTM Kit

Product Number:    2001, 2030

Application:    Isolation of genomic DNA from cells and tissues. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

      • 2001: 10 minipreps (use 100 ul AquaGenomic per 1 million culture cells)
      • 2030: 300 minipreps 

Kit Contents:   The AquaGenomic Kit includes the following items

      • 2001: 1 ml AquaGenomic Solution, User Manual
      • 2030: 30 ml AquaGenomic Solution, User Manual

Price:   

    • 2001: $10 each
    • 2030: $199 each

Ordering:    To order, please click the "BUY" button below.  


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MultiTarget Pharmaceuticals, LLC - All rights reserved