AquaGenomic is an aqueous reagent for genomic DNA extraction. It may be used to extract DNA from all types of specimens, from bacteria to animal tissues. The extraction protocols are simple, fast, and scalable. AquaGenomic is nontoxic and its lysate may be used for PCR directly without DNA purification. AquaGenomic can efficiently extract DNA from dried specimen swabs. It may facilitate the use of dried specimen swabs for low-cost biobanking, biosurveillance and epidemiological studies.
AquaGenomic is a multi-functional aqueous solution for genomic DNA isolation and purification. It achieves cell lysis, DNA extraction, and debris removal in a single step.
Add AquaGenomic solution to lyse the cells, extract the DNA, and precipitate the cell debris. The crude lysate may be used directly for PCR analysis without further DNA purification.
No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no multiple solutions and buffers.
No phenol, no chloroform, no isoamylacohol, no saturated butanol, no guanidine hydrochloride.
One AquaGenomic Kit can be used to isolate DNA from cultured cells, buccal swabs, blood, bacteria, animal or plant tissues. No need to purchase different kits for different starting materials.
One single AquaGenomic Kit does all: mini, midi, or maxi. No need to purchase separate mini, midi, or maxi kits.
About 5-10 ug of DNA can be isolated from 1-2 million cultured cells, 15 mg of feces, 1 ml of bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues.
AquaGenomic is priced at $199/kit for 300 minipreps, that is $0.66/miniprep or 1/4 of the price of other genomic DNA isolation kits.
Comparison of AquaGenomic and column-based genomic DNA purification
Genomic DNA extracted from different mouse tissues with AquaGenomic. Approximately 10 mg of tissue or 100 ul of blood were processed using the AquaGenomic Kit. Final genomic DNA was suspended in 50 ul deionized water. Five-microliter of each prep was run in a 1% agarose gel.
(Please refer to the Instruction Manual for DNA extraction from bacteria, tissues, soil, saliva, blood, etc.)
1. Harvest the Cells
Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 12,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: For convenience, cell culture may be mixed with 1 vol of AquaGenomic for DNA extraction without needing to pellet the cells.)
2. Extract the DNA
Add 100 ul of AquaGenomic to the cell pellet. Suspend and lyse the cells by vortex vigorously for 30-60 sec. Incubate at 22 °C for 5 min. (Optional: Incubate at 75 °C for 20 min for best DNA yield, DNase inactivation and RNA degradation.)
(Note: To use the lysate for PCR, dilute an aliquot of the lysate with 10-20 vol of water and then use 0.25 ul of the diluted lysate in a 25-ul PCR reaction.)
3. Pellet the DNA
Add 0.8 vol of 100% isopropanol (e.g., to 100 ul crude lysate, add 80 ul isopropanol) and vortex for 30-60 sec to mix well. Centrifuge at 12,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant.
4. Rinse the DNA
Gently fill up the tube with 70% ethanol from a squirt bottle, and then flip the tube to discard the ethanol solution. Repeat the 70% ethanol rinse once. Tap the tube on a paper towel to remove residual ethanol solution and leave it upside down for 5-10 min to air-dry the DNA pellet.
5. Solubilize the DNA
Add 100 ul of TE buffer or deionized water to the DNA pellet, pipette or vortex vigorously to suspend the DNA. Incubate at 22 °C for 15 min (Optional: To fully solubilize and recover the DNA, incubate at 65-75 °C for 15 min.). Centrifuge at 12,000 xg for 5 min to pellet the insoluble and transfer the clear DNA solution to a new tube.
Product Name: AquaGenomicTM Kit
Product Number: 2001, 2030
Application: Isolation of genomic DNA. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaGenomic Kit includes the following items
Ordering: To order, please click the "BUY" button below.
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