AquaGenomicTMa
multifunctional aqueous solution-based reagent for genomic DNA isolation and
purification. This single solution will lyse the cells, extract the
DNA, and precipitate the cell debris. The protocols are simple,
fast, and scalable. Approximately 5-10 ug
DNA can be
isolated from 1-2 million cultured cells, 1 ml of bacterial cultures,
5-10 mg of animal tissues, or 10-20 mg of plant tissues. AquaGenomic
may also be used to extract DNA from dry blood spots (DBS) or stains on
FTA or Guthrie
cards, cloth, papers, swabs and other forensic specimens with high
yield (~500 ng/6-mm punch). AquaGenomic is a user- and
environment-friendly reagent and it contains no chemical that is
harsher than SDS, a component of household detergents.
AquaGenomicis a multi-functional aqueous
solution for genomic DNA isolation and purification.
It achieves cell lysis, DNA extraction, and debris removal in
a single step.
Simple
Add AquaGenomic
solution to lyse the cells, extract the DNA, and precipitate
the cell debris, and then add isopropanol to precipitate the
purified genomic DNA.
Easy
No columns, no resins,
no slurries, no beads, no filters, no membranes, no needles,
no cartridges, no manifolds, no multiple solutions and buffers.
Nontoxic
No phenol, no chloroform, no isoamylacohol, no
saturated butanol, no guanidine hydrochloride.
Fast
Two minutes to lyse cells and extract
DNA, four minutes to pellet cell debris, two minutes
to pellet DNA. From cells or tissues, to DNA in ~15 minutes.
Pure
A260/A280 of the isolated genomic DNA is
1.6-1.8. The genomic DNA
is free from enzyme inhibitors.
Flexible
One AquaGenomic Kit can be used to isolate DNA from
cultured cells, buccal swabs, blood, bacteria, animal or plant
tissues. No need to purchase different kits for different starting
materials.
Scalable
One single AquaGenomic Kit does all: mini, midi, or
maxi. No need to purchase separate mini, midi, or maxi kits.
High Yield
About 5-10 ug of DNA can be isolated from from 1-2
million cultured cells, 15 mg of feces, 1 ml of
bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant
tissues.
Economic
AquaGenomic is priced at $199/kit for 300
minipreps, that is $0.66/miniprep or 1/4 of the price of
other genomic DNA isolation kits.
Comparison
Below is a comparison of AquaGenomic and a leading
competitor. You can easily see the advantages and benefits of using
AquaGenomic.
Competitor
Q
AquaGenomic
Isolation
Mechanism
Selective
Binding
Selective
Extraction
Isolation
Format
Solid
Support
Aqueous
Solution
Price
($/miniprep)
2.10-6.64
0.66
Proteases
Yes
No
Chaotropic
Salt
Yes
No
Multiple
Kits
Yes, mini, midi, maxi;
animal, plant, blood, swab, etc.
No,
a single kit for all sizes and materials
#
of Solutions in the Kit
6-7
1
Isolation
Time (min)
30
(mini)
60 (midi)
90
(maxi)
4-16
hours (tissue)
15 (mini)
15 (midi)
15 (maxi)
15 (tissue)
Yield
(ug DNA)
10 (mini)
60 (midi)
600
(maxi)
10 (mini)
100 (midi)
1000 (maxi)
Purity
(A260/A280)
1.7-1.9
1.6-1.8
Scalable
No
Yes
(100 ul AG Solution/million cells)
Comparison of genomic DNA
isolated from different mouse tissues by AquaGenomic. Approximately 10
mg of tissue or 100 ul of blood were processed using the AquaGenomic
Kit. Final genomic DNA was suspended in 50 ul deionized
water. Five-microliter of each prep was run in a 1%
agarose gel.
Brief Protocol
1. Harvest the Cells
Pellet
~0.5-2 million cultured cells in a 1.5-ml microfuge tube by
centrifugation at 14,000 xg for 60 sec. Aspirate or decant to
discard the supernatant.
2. Lyse the cells
Add
100 ul AquaGenomic solution (30
ml in the kit for 300 minipreps) to the cell pellet. Vortex vigorously for 60
sec to lyse the cells.
3. Remove the Debris
Centrifuge
the sample at 14,000 xg for 5 min to pellet the debris.
4. Pellet the DNA
Transfer the supernatant (~90 ul) to a new 0.5-ml
microfuge tube. Add 0.8 vol (~72 ul) of isopropanol and vortex to mix. Centrifuge at
14,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant and
rinse the DNA pellet with 70% ethanol twice. Suspend the DNA pellet in 100 ul
of TE buffer or deionized water. Centrifuge again to pellet any insoluble
material and transfer the DNA solution to a new tube.
Ordering Information
Product
Name: AquaGenomicTM Kit
Product Number:
2001, 2030
Application:
Isolation of genomic
DNA from cells and tissues. For in vitro research use only.
Size:
The kit is sufficient for the preparation of
2001:
10 minipreps (use 100 ul AquaGenomic for 1 million culture cells)
2030: 300
minipreps
Kit
Contents: The AquaGenomic Kit
includes the following items
2001: 1 ml AquaGenomic
Solution, User Manual
2030: 30 ml
AquaGenomic Solution, User Manual
Price:
2001: $10 each
2030: $199 each
Ordering:
To order, please click the "BUY"
button below.
