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AQUAGENOMIC

AquaGenomic is an aqueous reagent for genomic DNA extraction. It may be used to extract DNA from all types of specimens, from bacteria to animal tissues. The extraction protocols are simple, fast, and scalable. AquaGenomic is nontoxic and its lysate may be used for PCR directly without DNA purification. AquaGenomic can efficiently extract DNA from dried specimen swabs. It may facilitate the use of dried specimen swabs for low-cost biobanking, biosurveillance and epidemiological studies.

AG







Features

Innovative

AquaGenomic is a multi-functional aqueous solution for genomic DNA isolation and purification. It achieves cell lysis, DNA extraction, and debris removal in a single step.

Simple

Add AquaGenomic solution to lyse the cells, extract the DNA, and precipitate the cell debris. The crude lysate may be used directly for PCR analysis without further DNA purification.

Easy

No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no multiple solutions and buffers.

Nontoxic

No phenol, no chloroform, no isoamylacohol, no saturated butanol, no guanidine hydrochloride.

Flexible

One AquaGenomic Kit can be used to isolate DNA from cultured cells, buccal swabs, blood, bacteria, animal or plant tissues. No need to purchase different kits for different starting materials.

Scalable

One single AquaGenomic Kit does all: mini, midi, or maxi. No need to purchase separate mini, midi, or maxi kits.

High yield

About 5-10 ug of DNA can be isolated from 1-2 million cultured cells, 15 mg of feces, 1 ml of bacterial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues.

Economic

AquaGenomic is priced at $199/kit for 300 minipreps, that is $0.66/miniprep or 1/4 of the price of other genomic DNA isolation kits.


Comparison

Comparison of AquaGenomic and column-based genomic DNA purification

   Column-based  AquaGenomic
 Format  Solid support  Aqueous Solution
 Price ($/mini)  2.10-6.46  0.66
 Chaotropic salt  Yes  No
 Multiple kits  Yes, mini, midi, maxi; animal, plant, blood, etc.  No, one kit for all sizes and materials
 # of solutions  6-7  1
 Lysate for PCR  No  Yes
 Scalable  No  Yes


AG extraction

Genomic DNA extracted from different mouse tissues with AquaGenomic. Approximately 10 mg of tissue or 100 ul of blood were processed using the AquaGenomic Kit. Final genomic DNA was suspended in 50 ul deionized water. Five-microliter of each prep was run in a 1% agarose gel.







Brief Protocol

(Please refer to the Instruction Manual for DNA extraction from bacteria, tissues, soil, saliva, blood, etc.)


1. Harvest the Cells

Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 12,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: For convenience, cell culture may be mixed with 1 vol of AquaGenomic for DNA extraction without needing to pellet the cells.)
 
2. Extract the DNA

 Add 100 ul of AquaGenomic to the cell pellet. Suspend and lyse the cells by vortex vigorously for 30-60 sec. Incubate at 22 °C for 5 min. (Optional: Incubate at 75 °C for 20 min for best DNA yield, DNase inactivation and RNA degradation.)

(Note: To use the lysate for PCR, dilute an aliquot of the lysate with 10-20 vol of water and then use 0.25 ul of the diluted lysate in a 25-ul PCR reaction.)

3. Pellet the DNA

Add 0.8 vol of 100% isopropanol (e.g., to 100 ul crude lysate, add 80 ul isopropanol) and vortex for 30-60 sec to mix well. Centrifuge at 12,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant.

4. Rinse the DNA

Gently fill up the tube with 70% ethanol from a squirt bottle, and then flip the tube to discard the ethanol solution. Repeat the 70% ethanol rinse once. Tap the tube on a paper towel to remove residual ethanol solution and leave it upside down for 5-10 min to air-dry the DNA pellet.

5. Solubilize the DNA

Add 100 ul of TE buffer or deionized water to the DNA pellet, pipette or vortex vigorously to suspend the DNA. Incubate at 22 °C for 15 min (Optional: To fully solubilize and recover the DNA, incubate at 65-75 °C for 15 min.). Centrifuge at 12,000 xg for 5 min to pellet the insoluble and transfer the clear DNA solution to a new tube.








Order Information

Product Name:    AquaGenomicTM Kit 

Product Number:   2001, 2030

Application:    Isolation of genomic DNA. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

  • 2001: 10 minipreps (use 100 ul AquaGenomic per 1 million culture cells)
  • 2030: 300 minipreps

Kit Contents:   The AquaGenomic Kit includes the following items

  • 2001: 1 ml AquaGenomic solution, User Manual
  • 2030: 30 ml AquaGenomic solution, User Manual

Price:  

  • 2001: $10 each
  • 2030: $199 each
Ordering:    To order, please click the "BUY" button below. 

  


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