AquaPlasmidTM is a novel
aqueous solution-based kit for the isolation and purification
of plasmid DNA. The clear advantages of AquaPlasmid are its
low cost, simple protocol, and high DNA purity. It provides
more benefits to the users than any other existing plasmid
DNA isolation kit.
AquaPlasmidrepresents a breakthrough in plasmid
DNA isolation. It allows the isolation and purification of
plasmid DNA without the use of a solid support.
Simple
Its protocol is
similar to alkaline lysis protocol, except that the isolated DNA is
ultrapure. Cells are lysed, debris is precipitated,
and then plasmid DNA is precipitated.
Easy
No columns, no
resins, no slurries, no beads, no filters, no membranes, no
needles, no cartridges, no manifolds, no HPLC,
no ultracentrifuge.
Nontoxic
No phenol, no
chloroform, no saturated butanol, no ethidium bromide, no cesium
chloride, no guanidine hydrochloride, no buffer 1, 2, 3, 4,
..., or A, B, C, D, ...
Fast
One minute to
pellet the bacteria, two minutes to lyse the cells, five
minutes to pellet bacterial debris, five minutes to pellet
plasmid DNA.
Pure
Plasmid DNA
is essentially free of cellular impurities and enzyme
inhibitors. A260/A280 = 2.0. It is excellent for common
molecular biology applications.
Scalable
One single
AquaPlasmid Kit does all: mini, midi, maxi, and 96-well HTP; no need
to purchase separate mini, midi, maxi, and
HTP kits.
High
Yield
5-10, 50-100,
500-1000 ug of DNA can be isolated from 1 ml (mini), 10 ml
(midi), 100 ml (maxi)overnight bacterial
cultures.
Economic
AquaPlasmid kit is
priced at $199/kit for 500 minipreps, that is $0.40/miniprep or
approximately 1/3 the price of other plasmid DNA isolation
kits.
Comparison
Below is a
comparison of AquaPlasmid and a leading competitor. You can easily
see the advantages and benefits of using
AquaPlasmid.
Competitor Q
AquaPlasmid
Isolation
Mechanism
Selective Binding
Selective Extraction
Isolation Format
Solid
Support
Aqueous
Solution
Price ($/miniprep)
1.21
0.40
RNase
A
Yes
No
Guanidine
HCl
Yes
No
Scalable
No
Yes
# of Solutions in the
Kit
7
2
Isolation Time
(min)
30 (mini)
120 (midi)
180 (maxi)
20 (mini)
30 (midi)
30
(maxi)
Yield (ug DNA/ml
culture)
2-4 (LB)
Can't use
TB
2-5 (LB)
~10 (TB)
Purity
(A260/A280)
1.8-2.0
1.9-2.0
Sequencing
(Phred20)
~700
~900
Click to view a typical
sequencing chromatogram from AquaPlasmid isolated
DNA. Sequencing was done on an ABI3730 96-Capillary Sequencer using
4ul plasmid miniprep (100ul at 250 ng/ul).
Ordering
Information
Product
Name: AquaPlasmidTM Kit
Product
Number: 1001, 1030
Application:
Isolation of
plasmid DNA. For in vitro research use
only.
Size: The kit is sufficient
for the preparation of
1001: 15
minipreps
1030: 500
minipreps, or 50 midipreps, or 5
maxipreps
Kit
Contents: The AquaPlasmid Kit includes the
following items
1001: 0.5
ml AquaLysis Solution, 1 ml AquaPlasmid Solution, Instruction
Manual
1030: 15ml
AquaLysis Solution, 30 ml AquaPlasmid Solution,
Instruction Manual
Price:
1001: $10 each
1030: $199 each
Ordering:
To order, please click the "BUY"
button below.
