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     AquaPlasmid, AquaGenomic, & AquaRNA aqueous solutions for nucleic acid isolation and purification

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AquaPlasmidTM is a novel aqueous solution-based kit for the isolation and purification of plasmid DNA. The clear advantages of AquaPlasmid are its low cost, simple protocol, and high DNA purity. It provides more benefits to the users than any other existing plasmid DNA isolation kit.

 


  Features

Innovative

AquaPlasmid represents a breakthrough in plasmid DNA isolation. It allows the isolation and purification of plasmid DNA without the use of a solid support.

 

Simple

Its protocol is similar to alkaline lysis protocol, except that the isolated DNA is ultrapure. Cells are lysed, debris is precipitated, and then plasmid DNA is precipitated. 

Easy

No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no HPLC, no ultracentrifuge.

Nontoxic

No phenol, no chloroform, no saturated butanol, no ethidium bromide, no cesium chloride, no guanidine hydrochloride, no buffer 1, 2, 3, 4, ..., or A, B, C, D, ...

Fast

One minute to pellet the bacteria, two minutes to lyse the cells, five minutes to pellet bacterial debris, five minutes to pellet plasmid DNA.

Pure

Plasmid DNA is essentially free of cellular impurities and enzyme inhibitors. A260/A280 = 2.0. It is excellent for common molecular biology applications.

Scalable

One single AquaPlasmid Kit does all: mini, midi, maxi, and 96-well HTP; no need to purchase separate mini, midi, maxi, and HTP kits.

High Yield

5-10, 50-100, 500-1000 ug of DNA can be isolated from 1 ml (mini), 10 ml (midi), 100 ml (maxi)overnight bacterial cultures.

Economic

AquaPlasmid kit is priced at $199/kit for 500 minipreps, that is $0.40/miniprep or approximately 1/3 the price of other plasmid DNA isolation kits.

 


Comparison

Below is a comparison of AquaPlasmid and a leading competitor. You can easily see the advantages and benefits of using AquaPlasmid.

   Competitor Q  AquaPlasmid

Isolation Mechanism

Selective Binding

Selective Extraction

Isolation Format

Solid Support

Aqueous Solution

Price ($/miniprep)

1.21

0.40

RNase A

Yes

 No

Guanidine HCl

Yes

No

Scalable

No

Yes

# of Solutions in the Kit

7

2

Isolation Time (min)

30 (mini)

120 (midi)

180 (maxi)

20 (mini)

30 (midi)

30 (maxi)

Yield (ug DNA/ml culture)

 2-4 (LB)

Can't use TB

2-5 (LB)

~10 (TB)

Purity (A260/A280)

1.8-2.0

1.9-2.0

Sequencing (Phred20)

~700

~900

 Click trace.png to view a typical sequencing chromatogram from AquaPlasmid isolated DNA. Sequencing was done on an ABI3730 96-Capillary Sequencer using 4ul plasmid miniprep (100ul at 250 ng/ul).

 


Ordering Information

Product Name:    AquaPlasmidTM Kit

Product Number:   1001, 1030

Application:    Isolation of plasmid DNA. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

      • 1001: 15 minipreps
      • 1030: 500 minipreps, or 50 midipreps, or 5 maxipreps 

Kit Contents:   The AquaPlasmid Kit includes the following items

      • 1001: 0.5 ml AquaLysis Solution, 1 ml AquaPlasmid Solution, Instruction Manual
      • 1030: 15ml AquaLysis Solution, 30 ml AquaPlasmid Solution, Instruction Manual

Price:  

      • 1001: $10 each
      • 1030: $199 each

Ordering:    To order, please click the "BUY" button below.  

BUY


 

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