AquaPlasmidTM is a novel aqueous solution-based reagent for isolation and purification of plasmid DNA. It yields ~5-10 ug of plasmid DNA from a milliliter of overnight bacterial culture. The purified DNA has an A260/A280 ratio of 1.8-2.0 and A260/A230 ratio of 2.0-2.2. The extraction protocol is as simple as alkaline lysis and it can be carried out using an inexpensive personal picofuge. AquaPlasmid reagent does not contain guanidine hydrochloride, a not readily biodegradable chemical found in most plasmid DNA purification kits.
AquaPlasmid represents a breakthrough in plasmid DNA isolation. It allows the isolation and purification of plasmid DNA without using solid support, such as columns, resins, filters, and cartridges. It greatly reduces material and energy consumption in producing these products and the generation of plastic wastes in using these products.
The AquaPlasmid protocol is as simple as alkaline lysis: cells are lysed, debris is removed, and plasmid DNA is then precipitated. No buffer 1, 2, 3, 4, ..., or A, B, C, D, .... Furthermore, the entire extraction can be done without needing a high-speed centrifuge, just a personal picofuge will do, enabling plasmid DNA extraction in high school science classrooms or resource-limited laboratories; and you could even do 96-well HTP minipreps with a speed-vac centrifuge.
No phenol, no chloroform, no saturated butanol, no ethidium bromide, no cesium chloride, no guanidine hydrochloride, or any other toxic component.
The isolated plasmid DNA is essentially free of cellular impurities and enzyme inhibitors. A260/A280 = 1.8-2.0 and A260/A230 = 2.0-2.2. Excellent for all downstream applications.
One single AquaPlasmid Kit does all: mini, midi, maxi, and 96-well HTP; no need to purchase separate and bulky mini, midi, maxi, and HTP kits.
A 15-ml AquaPlasmid kit costs $199, sufficient for 300 minipreps, that is only $0.66/miniprep. It is the most inexpensive plasmid DNA isolation kit on the market.
Below is a comparison of AquaPlasmid and column-based competitors. You can see the advantages and benefits of using AquaPlasmid.
1.Harvest the Cells
Centrifuge 2 ml overnight culture at 3,000-14,000 xg at room temperature for 1 min to pellet the bacteria. Aspirate to remove the culture medium as completely as possible.
2. Lyse the Cells
Add 80 ul of deionized water to the cell pellet. Vortex vigorously to fully resuspend the cells. Add 100 ul of AquaLysis solution to the cell suspension. Vortex vigorously to mix the contents well. Incubate at room temperature for 5 min.
3. Remove the Debris
Add 50 ul of AquaPlasmid solution (15 ml in the kit for 300 minipreps) to the crude lysate. Touch-vortex (a few seconds on and then a few seconds off) at top speed to mix the contents. Centrifuge at 3,000-14,000 xg for 5 min to pellet the debris.
4. Pellet the Plasmid DNA
Transfer the clear lysate (210 ul) to a new 0.5-ml microfuge tube. Add 1/3 vol (70 ul) of isopropanol to the lysate. Vortex for 20-30 sec to mix well. Centrifuge at 3,000-14,000 xg for 5 min to pellet the plasmid DNA. Flip the tube to discard the supernatant. Fill the tube with 70% ethanol, and then flip to discard the ethanol. Repeat the ethanol rinse once. Air-dry the DNA pellet (nearly invisible) for ~5 min. Suspend the DNA in 50 ul of deionized water. The expected DNA yield is 5-10 ug per ml of overnight culture. The DNA purity is ~1.8-2.0 at 260/280 and ~2.0-2.2 at 260/230.
Product Name: AquaPlasmidTM Kit
Product Number: 1001, 1015
Application: Isolation of plasmid DNA. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaPlasmid Kit includes the following items
Ordering: To order, please click the "BUY" button below.
Copyright © 2013 MultiTarget Pharmaceuticals, LLC - All rights reserved