AquaPreserveÔ
is an aqueous solution based preservative with DNA, RNA, and protein
extraction functionality. It may be used to streamline biospecimen
(such as whole blood) collection, stabilization, transport, storage,
and DNA, RNA, and protein extraction. It simplifies biospecimen
processing, reduces processing heterogeneity, and eliminates
artificial pre-analytical changes in a specimen. Whole blood samples
can be collected in standard EDTA vacutainers, aliquoted, and stored
at -80 °C or they may be stabilized in
AquaPreserveÔ,
stored and shipped at ambient temperatures, and subsequently DNA,
RNA, and proteins can be extracted from the blood samples with
AquaPreserveÔ
without using additional extraction kits.
Blood is the most
collected and stored biospecimen, but there are only a
few Google hits of "RNA from frozen whole blood," not because
there is no need, but because there is no way. Existing
RNA extraction kits cannot extract intact RNA from
frozen whole blood.
Now you can extract
total RNA from fresh and frozen whole blood with
AquaPreserve: mix 2 volumes of AquaPreserve to 1 volume of
blood, pellet proteins with 1/3 volumes of ProSink, and then
precipitate DNA and RNA with 1 volume of isopropanol. Simple
and scalable.
Collect blood in
EDTA vacutainers and store them at -80°C.
Use no RNA
preservative-containing PAXgene or Tempus tubes.
Extract RNA from population
or disease-targeted blood samples for prospective
studies.
Extract RNA from surplus
or archived blood samples for retrospective
studies.
Recover not only cellular but also
cell-free DNA and RNA in whole
blood.
Require no WBC
fractionation or RBC lysis or protease digestion of
hemoglobin.
Extract DNA,
RNA, and proteins from both fresh and frozen whole
blood.
Use no additional and different DNA,
RNA, and protein extraction kits.
Preservation and
extraction of blood DNA and RNA with
AquaPreserve. Frozen human ACD blood was thawed in 2 vol of
AquaPreserve. Aliquots of the AquaPreserve lysed blood were
stored at -80, 22, and 37°C for 7 days. DNA and RNA were
recovered from the cleared lysate by isopropanol
precipitation. One tenth of the total DNA/RNA from 200 ul of
whole blood was digested with DNase I and electrophoresized in
a 0.6% non-denaturing agarose gel.
Features
Preservation and
Extraction
There
is a significant amount of heterogeneity in the methods used to
collect, process, store, and extract biospecimens for molecular
analyses. Improper handling of biospecimens may artificially
introduce molecular changes in the biospecimens, often without the
knowledge of the researchers, which in turn can lead to false
research findings or wrong diagnoses.
By combining biospecimen preservation and biomolecule extracion with
AquaPreserve, it may be possible to standardize and streamline the
entire pre-analytical biospecimen workflow, from
biospecimen collection, stabilization, transport, storage, to
biomolecule extraction, and thus, prevent or reduce artificially
introduced variabilities in a
biospecimen.
Stabilization without
Crosslinking
Formalin
is the most commonly used preservative for stabilizing biospecimens.
However, it causes
significant damages to the biomolecules by crosslinking DNA, RNA,
and proteins in the preserved tissues and could compromise
downstream molecular analyses. AquaPreserve stabilizes
biospecimen by inactivating degradative enzymes without causing
biomolecule crosslinking.
Extracting DNA, RNA, and
proteins without Using Other Extraction
Kits
With AquaPreserve, you don't need to
worry about which kits to use for DNA, RNA, and protein
extraction. They can all be accomplished with this single solution.
Simply homogenize the specimen in AquaPreserve to extract DNA, RNA,
and proteins, and then precipitate the DNA/RNA with isopropanol
and precipitate the proteins with acetone.
It would not only save processing time, labor, and money, but most
importantly also prevent pre-analytical variables from using
different extraction kits.
Comparison
Comparison
of Techniques for Biospecimen
Preservation
Formalin
R*later
AquaPreserve
Mechanism of Stabilization
Cross-linking
High
salt dehydration
Enzyme
inactivation
Add
to Frozen Specimens
No
No. Require pre-soaking
Yes
Storage
Temperature
22 °C
22 °C
22 to
-80 °C
Damage
to Specimen
Yes
No
No
Inactivation
of Pathogens
Yes
No
Yes
Offensive
Odor
Yes
No
No
DNA,
RNA, and protein Extraction
No
No
Yes
Gross
anatomy of liver tissues preserved in AquaPreserve and
PBS.
Fresh mouse liver tissues (~100 mg) were submerged in 0.2 ml
AquaPreserve (left) or PBS (right) and stored at 22°C for 7 days. As
seen, liver tissues preserved in AquaPreserve looked fresh, remained
intact, and wedged in the tube, while liver tissues stored in PBS
became highly degraded and sank to the bottom of the
tube.
Ordering
Information
Product
Name: AquaPreserve Kit
Product
Number: 8001, 8030
Application:Biospecimen preservation and extraction.
For in vitro research use only.
Size: The
kit is sufficient for the preparation of
8001: 1ml for 1
extraction
8030: 30 ml for 30
extractions
(Please keep in mind that AquaPreserve is
scalable. Depending on your specimen type and need, you may use
as little as 100 ul of AquaPreserve to preserve and
extract 5 mg of tissues and obtain 10-50 ug of total DNA/RNA.
Therefore, 1 ml AquaPreserve could give you 10 extractions and 30
ml would give you 300
extractions.)
Kit Contents: The
AquaPreserve Kit includes the following
items
8001: 1
ml AquaPreserve Solution, Instruction
Manual
8030: 30
ml AquaPreserve Solution, Instruction
Manual
Price:
8001: $10 each
8030: $199 each
Ordering:
To order, please
click the "BUY"
button below.
