blood RNA,RNA extraction,biospecimen,DNA extraction,cell viability ,preservative,random mutagenesis rocky
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AQUAPRESERVE

AquaPreserve is a multifunctional reagent for DNA/RNA/protein preservation and extraction. It may be used to streamline biospecimen collection, stabilization, transport, storage, distribution, and DNA/RNA/protein extraction. By streamlining the entire biospecimen workflow, AquaPreserve can reduce pre-analytical variability, increase data reproducibility and reliability. AquaPreserve can extract total DNA/RNA/proteins from blood, saliva and other liquid biospecimens; it may recover both cellular and cell-free circulating DNA/RNA/proteins in a specimen, and thus maximizing the scientific value and utilities of the biospecimens. AquaPreserve is the only reagent that can extract intact RNA from frozen whole blood samples collected in common anticoagulants.

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Features

Streamline biospecimen pre-analytical workflow

AquaPreserve not only preserves but also extracts DNA/RNA/proteins from the biospecimen. It can be used to streamline the entire pre-analytical workflow, from specimen collection (immediately arrests gene expression and stabilizes the DNA/RNA/proteins) to DNA/RNA/protein extraction (does not use any other DNA, RNA, and protein extraction kits). Therefore, it reduces biospecimen pre-analytical variability, prevents sample cross-contamination, increases detection specificity, and improves data reproducibility and reliability.

Safeguard biospecimens during their transportation and storage

AquaPreserve/ProSink-stabilized specimens may be stored at 4 and 22 °C for weeks without causing RNA degradation. AquaPreserve serves as a safeguard against unexpected cold-chain disasters during shipping and storage, and makes it feasible to repeatedly thaw the frozen the specimens for QC/QA and for distribution to researchers.
 
Maximize the scientific value of the biospecimens

Presently you probably only store cellular DNA extracted from fresh blood and you don't have a reliable method to extract RNA from frozen whole blood collected in common anticoagulants (not in specialized PAXgene or Tempus RNA tubes). With AquaPreserve, you not only can extract blood DNA but also blood RNA, not only cellular DNA/RNA/proteins but also cell-free circulating DNA/RNA/proteins, from not only fresh but also archived frozen whole blood. It allows you to do more researches and generate more data previously impossible from your precious specimens.

Reduce the overall cost of biobanking

AquaPreserve enables the freezing of leftover blood samples obtained from routine testing for later DNA/RNA/protein extraction, therefore, opens up an unlimited and inexpensive biospecimen resource. AquaPreserve-stabilized the blood biospecimens may be shipped and stored at 4 or 22 °C for years without causing DNA degradation, therefore, reducing the energy consumption. Additionally, AquaPreserve extracts DNA/RNA/proteins from the specimens without needing other DNA, RNA, and protein extraction kits


Comparison

Comparison of Techniques for Biospecimen Preservation

 

 Formalin

 R*later

 AquaPreserve

Stabilization Mechanism

Cross-linking High salt dehydration Enzyme inactivation

Damage to Specimen

Yes No

 No

Inactivation of Pathogens

Yes

No

Yes

Offensive Odor

Yes

No

No

DNA, RNA, and Protein Extraction

No

No Yes
DNase treated

Frozen human ACD blood was thawed in 1 vol of AquaPreserve. Aliquots of the AquaPreserve lysed blood were mixed with ProSink and stored at -80, 22, and 37°C for 7 days. DNA and RNA were recovered from the cleared lysate by isopropanol precipitation. One tenth of the total DNA/RNA from 200 ul of whole blood was digested with DNase I and electrophoresized in a 0.8% non-denaturing agarose gel.







Brief Protocol

1. Lyse the blood cells

Add 0.25 ml of AquaPreserve to 0.25 ml of fresh or frozen whole blood in a 1.5-ml microfuge tube. Vortex or shake to mix well or until the frozen blood sample is completely thawed in AquaPreserve (Do not thaw the frozen blood without mixing with AquaPreserve or the RNA will be degraded during blood thawing). Incubate at 22 °C for 15 min. Shake and vortex vigorously to break up the blood clot and centrifuge at 14,000 xg for 5 min to pellet the debris.

2. Remove the proteins

Add 0.125 ml of ProSink (#9030, order separately) to the lysate. Invert the tube and touch vortex a few times to mix the lysate and ProSink without dislodging the debris pellet. Incubate at 22 °C for >30 min (Blood DNA is now stable at 4-22 °C for months, and blood RNA is stable at 4 °C for 2 weeks and 22 °C for 7 days). Centrifuge at 14,000 xg for 5 min to pellet the proteins.

3. Pellet the DNA/RNA

Transfer the supernatant (~0.7 ml) to a 1.5-ml microfuge tube preloaded with 0.7 vol (~0.49 ml) of isopropanol and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA. Decant to discard the supernatant (or save it for small molecule analysis) and rinse the DNA/RNA pellet with 50% isopropanol twice. Air dry the DNA/RNA pellet. Suspend the DNA/RNA pellet in 100 ul of TE buffer or deionized water.

Use the volume ratio of 1:1:0.5 (blood:AquaPreserve:ProSink) for other extraction scales

    Micro Mini Midi Maxi
Blood (ul) 50 250 2,000 4,000
AquaPreserve (ul) 50 250 2,000 4,000
ProSink (ul)  25 125 1,000 2,000
Centrifuge tubes 0.6-ml 1.5-ml 15-ml 15-ml
DNA yield (ug) 2-3 12-15 100-130 200-250
RNA yield (ng) 50 * 250 2,000 4,000
Extractions per 60ml AquaPreserve 1,200 240 30 15
Price per extraction ($) 0.21 1.03 8.27 16.53

* This is the RNA yield from human blood. For mouse blood, RNA yield may be 2500 ng/50 ul blood.







Order Information

Product Name:    AquaPreserveTM Kit 

Product Number:   8001, 8060

Application:    Biospecimen preservation and extraction. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

  • 8001: 4 DNA/RNA minipreps (use 0.25 ml AquaPreserve for 0.25 ml blood)
  • 8060: 240 DNA/RNA minipreps

Kit Contents:   The AquaPreserve Kit includes the following items

  • 8001: 1 ml AquaPreserve solution, User Manual
  • 8060: 60 ml AquaPreserve solution, User Manual

Price:  

  • 8001: $10 each
  • 8060: $199 each
Ordering:    To order, please click the "BUY" button below (order ProSink #9030 and ProMelt #1115 separately). 

  


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