blood RNA,RNA extraction,biospecimen,DNA extraction,cell viability ,preservative


 
   AquaPlasmid, AquaGenomic, & AquaRNA aqueous solutions for nucleic acid isolation and purification

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AquaPreserve

AquaPreserveTM is an aqueous solution-based preservative with DNA, RNA, and protein extraction functionality. It may be used to streamline blood sample collection, stabilization, transport, storage, and DNA, RNA, and protein extraction. The extraction protocols are simple. There is no need to remove the plasma or lyse the RBC or fractionate the blood cells prior to extraction. Cell-free DNA, RNA, and proteins will not be lost and gene expression profiles will not be altered artificially by in vitro separation of blood components and cells. Most uniquely, AquaPreserve can be used to extract intact total RNA from existing frozen blood samples collected in regular anticoagulants, thus enabling the use of surplus and archived frozen blood samples collected during routine medical care and clinical trials for blood RNA research

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Finally, total RNA can be extracted from frozen whole blood! 

Blood is the most collected biospecimen, however, there is no RNA kit for extracting intact RNA from archived frozen whole blood collected in regular anticoagulants.

With AquaPreserve, you can extract total RNA from fresh or frozen whole blood: (1) lyse whole blood with AquaPreserve, (2) pellet hemoglobin with ProSink, and then (3) precipitate DNA and RNA with isopropanol.

  • Collect blood in EDTA vacutainers and store them at -80°C.
  • Use no RNA preservative-containing PAXgene or Tempus tubes.
  • Extract RNA from population or disease-targeted blood samples for prospective studies.
  • Extract RNA from surplus or archived blood samples for retrospective studies.
  • Recover not only cellular but also cell-free DNA and RNA in whole blood.
  • Require no WBC fractionation or RBC lysis or protease digestion of hemoglobin.
  • Extract DNA, RNA, and proteins from both fresh and frozen whole blood.
  • Use no additional and different DNA, RNA, and protein extraction kits.

dnasei treated blood rna2.png

Preservation and extraction of blood DNA and RNA with AquaPreserve. Frozen human ACD blood was thawed in 2 vol of AquaPreserve. Aliquots of the AquaPreserve lysed blood were stored at -80, 22, and 37°C for 7 days. DNA and RNA were recovered from the cleared lysate by isopropanol precipitation. One tenth of the total DNA/RNA from 200 ul of whole blood was digested with DNase I and electrophoresized in a 0.8% non-denaturing agarose gel.

 

 


Features

 

Compatible with conventional blood draws

 

AquaPreserve is compatible with anticoagulated blood samples collected in routine medical care and clinical trials. The blood samples can be used for other clinical tests and only an aliquot of the extra or leftover blood samples are saved in AquaPreserve for DNA and RNA extraction

Safeguard the blood samples during long-term storage

AquaPreserve-stabilized blood sample can be stored long-term at -80 °C without risking RNA degradation due to accidental blood thawing during storage, such as power outage or freezer breakdownUnlike plain frozen blood, AquaPreserve-stabilized blood sample can be repeatedly thawed to give out aliquots for distribution or experiments, a significant advantage and advancement in blood biobanking.
 

Extracting DNA, RNA, and proteins without using other extraction kits

 

AquaPreserve not only stabilizes but also extracts DNA, RNA, and proteins without using additional extraction kits. It streamlines blood collection, transport, storage, and DNA/RNA/protein extraction, reducing pre-analytical variation and processing cost.  

 

 

 


Comparison

Comparison of Techniques for Biospecimen Preservation

 

 Formalin

 R*later

 AquaPreserve

Mechanism of Stabilization

Cross-linking

High salt dehydration

Enzyme inactivation

Add to Frozen Specimens

No

No. Require pre-soaking

Yes

Storage Temperature

22 °C

22 °C

22 to -80 °C

Damage to Specimen

Yes

No

 No

Inactivation of Pathogens

Yes

No

Yes

Offensive Odor

Yes

No

No

DNA, RNA, and protein Extraction

No

No

Yes

 
 
apv_pbs.png

 Gross anatomy of liver tissues preserved in AquaPreserve and PBS. Fresh mouse liver tissues (~100 mg) were submerged in 0.2 ml AquaPreserve (left) or PBS (right) and stored at 22 °C for 7 days. As seen, liver tissues preserved in AquaPreserve looked fresh, remained intact, and wedged in the tube, while liver tissues stored in PBS became highly degraded and sank to the bottom of the tube.

 

 


Brief Protocol

1. Lyse the blood cells

Add 0.25 ml of AquaPreserve to 0.25 ml of fresh or frozen whole blood in a 1.5-ml microfuge tube. Vortex or shake to mix well or until the frozen blood sample is completely thawed in AquaPreserve (Do not thaw the frozen blood without mixing with AquaPreserve or the RNA will be degraded during blood thawing. However, if you are only interested in blood DNA extraction, the blood sample may be thawed, aliquoted and then mixed with AquaPreserve.).

2. Remove the proteins

Add 0.125 ml of ProSink (#9030) to the AquaPreserve lysed blood. Vortex for 1 min to mix well and incubate at 22 °C for >30 min (Blood DNA is now stable at 4-22 °C for a year, and blood RNA is now stable at -80 °C forever, 4 °C for months, 22 °C for 2 weeks, and 37 °C for 2 days.). Centrifuge at 3,500-14,000 xg for 10 min to pellet the proteins.

3. Pellet the DNA/RNA

Pour the supernatant (~0.8 ml) to a 1.5-ml microfuge tube preloaded with 0.8 vol (~0.64 ml) of isopropanol and vortex to mix. Centrifuge at 3,500-14,000 xg for 10 min to pellet the DNA/RNA. Decant to discard the supernatant and rinse the DNA/RNA pellet with 70% ethanol twice. Suspend the DNA/RNA pellet in 50 ul of TE buffer or deionized water. 

Use the volume ratio of 1:1:0.5 (blood:AquaPreserve:ProSink) for other extraction scales

    Micro Mini Midi Maxi
Blood (ul) 50 250 2,000 4,000
AquaPreserve (ul) 50 250 2,000 4,000
ProSink (ul) 25 125 1,000 2,000
Centrifuge tubes 0.6-ml 1.5-ml 15-ml 15-ml
DNA yield (ug) 2-3 12-15 100-130 200-250
RNA yield (ng) 50* 250 2,000 4,000
Extractions per #8060 and #9030 1,200 240 30 15
Price per extraction ($) 0.21 1.03 8.27 16.53

* This is the RNA yield from human blood. For mouse blood, RNA yield may be 2.5 ug/50 ul blood.


Ordering Information

Buy-1-Get-1-Free Promotional Sales!

We will add a free unit of every AquaPreserve and ProSink ordered as a thank-you to our customers.

Product Name:    AquaPreserve Kit

Product Number:   8001, 8060

Application:   Biospecimen preservation and extraction. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

      • 8001: 4 DNA/RNA minipreps (use 0.25 ml AquaPreserve for 0.25 ml blood)
      • 8060: 240 DNA/RNA minipreps

Kit Contents:   The AquaPreserve Kit includes the following items

      • 8001: 1 ml AquaPreserve Solution, Instruction Manual
      • 8060: 60 ml AquaPreserve Solution, Instruction Manual

Price:  

      • 8001: $10 each
      • 8060: $199 each

Ordering:    To order, please click the "BUY" button below (order ProSink #9030 separately).  

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