blood RNA,RNA extraction,biospecimen,DNA extraction,protein extraction,preservative


 
   AquaPlasmid, AquaGenomic, & AquaRNA       aqueous solutions for nucleic acid isolation and purification

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AQUARNA

 

AquaRNATM is a multifunctional aqueous solution for DNA, RNA, and protein extraction. It functions to lyse the cells, inactivate RNases, and extract DNA, RNA, and proteins simultaneously. DNA and RNA are recovered from the cell lysate by isopropanol precipitation, while proteins are recovered from the cell lysate by acetone precipitation. AquaRNAÔ enables concurrent isolation of DNA, RNA, and proteins from the same specimen for analyses without using different DNA, RNA, and protein extraction kits. Additionally, AquaRNATM can be used to inactivate and remove contaminating RNases in purified RNA samples to ensure RNA integrity and stability.

 

 


  Features

 

RNA myth

AquaRNA will change the way you think and work with RNA by simply inactivating and removing endogenous contaminating RNases in your purified RNA samples.

  • "RNA is very unstable and must be stored at -80 °C." - Not true, if endogenous contaminating RNases are removed, RNA samples can be stored at 4 °C without degradation.  
  • "RNA work must be done at designated RNA-only workbench, use RNA-only equipment and RNase-free consumables." - This is unnecessary, just don't let your fingers, with or without gloves, touch the inside of your RNA tube, your chance of getting RNA degradation by using regular reagents, solutions, tubes, and tips is slim to none.
  • "I am always anxious until I see the results of my RNA experiments." - If the RNA is not contaminated with residual RNases, you will find that RNA is no longer difficult to work with and you will get consistent and reproducible results every time.

RNase Decontamination

 

AquaRNA can be used to treat purified RNA that is contaminated with RNases. It can also be used to treat plasmid DNA or in vitro transcribed RNA to remove any contaminating RNases to ensure successful in vitro transcription and translation.

 

Not only for RNA

 

AquaRNA can extract DNA, RNA, and proteins from the same biological samples. In addition to maximizing the value of your invaluable biospecimens, you might uncover new correlations and new differences by analyzing DNA, RNA, and proteins at the same time. 

 

Simple

Due to the multifunctionality of the AquaRNA solution, its protocol is very simple. After cell lysis, DNA/RNA are precipitated with isopropanol and proteins are precepitated with acetone. No columns, no resins, no cartridges, and no ultracentrifuge are needed.

No phenol and chloroform

AquaRNA extracts DNA/RNA/proteins much like the famous Trizol® (Invitrogen), but it doesn't use phenol and chloroform.

Scalable

One single AquaRNA Kit does all: mini, midi, maxi, and HTP; no need to purchase separate mini, midi, maxi, and HTP kits.

High Yield

Up to 50 ug of DNA, 25 ug of RNA, and 1500 ug of proteins can be isolated from 2.5 million mammalian cells or 25 mg animal tissue. Furthermore, you will no longer have the problem of getting the protein pellet into solution as you encounter with Trizol.

 

 


Comparison

 

 

 Competitor Q

 AquaRNA

Isolation Mechanism

Selective Binding

Selective Extraction

Isolation Format

Solid Support

Aqueous Solution

Price ($/miniprep)

3.84

0.66

Mercaptoethanol

Yes

 No

Scalable

No

Yes

# of Solutions in the Kit

4

1

Hands-on Time (min)

30

30

Yield (ug RNA/mil cells)

10-20

10-20

RNase Contamination

 ?

No

Recover Small RNAs

ar vs q.jpg

No

Yes

Co-Purify DNA/Proteins

Trace

Yes

 

rna gel for web3.jpg

Comparison of total RNA isolated from different sources by AquaRNA. Bacterial culture (0.5 ml), human cultured cells (500,000 cells), and rat liver tissue (20 mg) were processed using the AquaRNA Kit. Final DNA/RNA pellet was suspended in 100 ul DEPC water. Five-microliter of each prep treated with or without DNase I was run in a non-denaturing 0.5% native agarose gel, showing the DNA, 28S (23S), 18S (16S), and 5S RNA bands.

bioanalyzer.jpg


Ordering Information

Product Name:    AquaRNATM Kit

Product Number:   5001, 5030

Application:    Isolation of DNA/RNA/proteins. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

      • 5001: 1ml for 10 minipreps
      • 5030: 30 ml for 300 minipreps

Kit Contents:   The AquaRNA Kit includes the following items

      • 5001: 1 ml AquaRNA Solution, Instruction Manual
      • 5030: 30 ml AquaRNA Solution, Instruction Manual

Price:  

      • 5001: $10 each
      • 5030: $199 each

Ordering:    To order, please click the "BUY" button below.  

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