AquaRNA is a multifunctional aqueous solution-based reagent for DNA, RNA, and protein extraction. This single solution will lyse the cells, inactivate degradative enzymes, and extract DNA, RNA, and proteins. DNA and RNA are recovered from the cell lysate by isopropanol precipitation, while proteins remain soluble in the AquaRNA-isopropanol solution and can be recovered by acetone precipitation. AquaRNA enables concurrent isolation of DNA, RNA, and proteins from the same specimen without using different DNA, RNA, and protein extraction kits.
Inactivate and remove endogenous RNases
AquaRNA will change the way you work with RNA by killing endogenous RNases in your RNA samples.
After cell lysis, DNA/RNA are precipitated with isopropanol and proteins are precipitated with acetone.
One AquaRNA Kit does all. No need to purchase separate mini, midi, maxi, and HTP kits.
Not only for RNA
Extract DNA, RNA, and proteins from the same specimens to maximize the value of your precious samples.
No phenol and chloroform
Extracts DNA/RNA/proteins without using the toxic phenol and chloroform.
Comparison of AquaRNA and column-based RNA purification
DNA/RNA was extracted from bacterial culture, mammalian culture, and rat liver tissue with AquaRNA, and treated with or without DNase I. Shown are the DNA, 28S (23S), 18S (16S), and 5S RNA bands.
1. Harvest the Cells
Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 14,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: Centrifuging to pellet the cells may not be needed as long as 1 vol of AquaRNA is mixed with 1 vol of cell suspension for DNA/RNA extraction.)
2. Extract the DNA/RNA
Add 100ul AquaRNA solution (30 ml in the kit for 300 minipreps) to the cell pellet (or equal volume of cell suspension). Vortex vigorously for 60 sec to lyse the cells.
3. Pellet the DNA/RNA
Add 0.7 vol (~80 ul) of isopropanol to the crude lysate and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA. Decant to discard the supernatant (Note: Proteins remain in the isopropanol supernatant and can be recovered by precipitation in 4 vol of acetone.) and rinse the DNA/RNA pellet with 70% ethanol twice. Suspend the DNA/RNA pellet in 100 ul of TE buffer or deionized water. Centrifuge again to pellet any insoluble material and transfer the DNA/RNA solution to a new tube. Remove DNA in the DNA/RNA prep by DNase I digestion.
Product Name: AquaRNATM Kit
Product Number: 5001, 5030
Application: Isolation of DNA, RNA and proteins. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaRNA Kit includes the following items
Ordering: To order, please click the "BUY" button below.
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