AquaRNA will change the way you think and work with RNA by simply inactivating and removing endogenous contaminating RNases in your RNA samples.
AquaRNA may be used to remove contaminating RNases from purified RNA or remove contaminating RNases in purified plasmid DNA for in vitro transcription.
Not only for RNA
AquaRNA can extract DNA, RNA, and proteins from the same biological samples concurrently and allow you to maximize the value of your biospecimens.
Due to the multifunctionality of the AquaRNA solution, its protocol is very simple. After cell lysis, DNA/RNA are precipitated with isopropanol and proteins are precipitated with acetone. No columns are needed.
No phenol and chloroform
DNA/RNA/proteins without using phenol
and chloroform. You will not have problem
solubilizing the protein pellet as you often encounter using organic
One AquaRNA Kit does all. No need to purchase separate mini, midi, maxi, and HTP kits.
Total RNA was extracted from an E. coli culture and the RNA integrity was measured with Agilent 2100 Bioanalyzer.
DNA/RNA was extracted from bacterial culture, mammalian culture, and rat liver tissue with AquaRNA, and treated with or without DNase I. Shown are the DNA, 28S (23S), 18S (16S), and 5S RNA bands.
(Please refer to the Instruction Manual for DNA/RNA/protein extraction from bacteria, cultured cells, animal tissues, and plant tissues, etc.)
Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 14,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: Centrifuging to pellet the cells may not be needed as long as 1 vol of AquaRNA is mixed with 1 vol of cell suspension for DNA/RNA extraction.)
2. Extract the DNA/RNA
Add 100 ul AquaRNA solution (30 ml in the kit for 300 minipreps) to the cell pellet (or equal volume of cell suspension). Vortex vigorously for 60 sec to lyse the cells.
3. Pellet the DNA/RNAAdd 0.8 vol (~100 ul) of isopropanol to the crude lysate and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA. Decant to discard the supernatant (Note: Proteins remain in the isopropanol supernatant and can be recovered by precipitation in 4 vol of acetone.) and rinse the DNA/RNA pellet with 70% ethanol twice. Suspend the DNA/RNA pellet in 100 ul of TE buffer or deionized water. Centrifuge again to pellet any insoluble material and transfer the DNA/RNA solution to a new tube. Remove DNA in the DNA/RNA prep by DNase I digestion.
Product Name: AquaRNATM Kit
Product Number: 5001, 5030
Application: Isolation of DNA/RNA/proteins. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaRNA Kit includes the following items
Ordering: To order, please click the "BUY" button below.
Copyright © 2013 MultiTarget Pharmaceuticals, LLC - All rights reserved