AquaRNATMis
a multifunctional aqueous solution-based reagent for DNA, RNA, and
protein extraction. This single solution will lyse the cells,
inactivate degradative enzymes, and extract DNA, RNA, and proteins. DNA
and RNA are recovered from the cell lysate by isopropanol
precipitation, while proteins remain soluble in the isopropanol
solution and can be recovered by acetone precipitation. AquaRNA enables
concurrent isolation of DNA, RNA, and proteins from the same specimen
without using different DNA, RNA, and protein extraction kits.
AquaRNA will change the way you
think and work with RNA by simply inactivating and removing endogenous
contaminating RNases in your RNA samples.
"RNA is very unstable and must
be stored at -80 °C." - Not true, when endogenous contaminating RNases are removed, RNA samples can be
stored at 4 °C.
"RNA work must be done at
designated RNA-only workbench, use RNA-only equipment and RNase-free
consumables." - Unnecessary, just don't let your
fingers, with or without gloves, touch the inside of your RNA tube,
your chance of getting RNA degradation by using regular reagents,
solutions, tubes, and tips is slim to none.
"I am always anxious until I see the
results of my RNA experiments."
- If the RNA sample is not contaminated with endogenous RNases, you will get consistent and reproducible results every
time.
RNase
Decontamination
AquaRNA
can be used to treat purified RNA that is contaminated with
RNases. It can also be used
to treat plasmid DNA to
remove any contaminating RNases to ensure successful in vitro
transcription and translation.
Not only for RNA
AquaRNA
can extract DNA, RNA, and proteins from the same biological samples concurrently. In
addition to maximizing the value of your biospecimens, you might uncover new correlations by
analyzing DNA, RNA, and proteins at the same time.
Simple
Due to the multifunctionality of
the AquaRNA solution, its protocol is very simple. After cell lysis,
DNA/RNA are precipitated with isopropanol and proteins are precipitated
with acetone. No columns, no resins,
no cartridges, and no ultracentrifuge are needed.
No phenol and chloroform
AquaRNA extracts DNA/RNA/proteins without
using phenol and chloroform. You will no longer have the problem of getting the
protein pellet into solution as you often encounter using organic extraction.
Scalable
One single AquaRNA Kit does all: mini, midi, maxi,
and HTP; no need to purchase separate mini, midi, maxi, and
HTP kits.
High Yield
Up to 50 ug of DNA, 25 ug of RNA, and 1500
ug of proteins can be isolated from 2.5 million
mammalian cells or 25 mg animal tissue.
Comparison
Competitor
Q
AquaRNA
Isolation
Mechanism
Selective
Binding
Selective
Extraction
Isolation
Format
Solid
Support
Aqueous
Solution
Price
($/miniprep)
3.84
0.66
Mercaptoethanol
Yes
No
Scalable
No
Yes
#
of Solutions in the Kit
4
1
Hands-on Time
(min)
30
30
Yield
(ug RNA/mil cells)
10-20
10-20
RNase
Contamination
?
No
Recover Small RNAs
No
Yes
Co-Purify
DNA/Proteins
Trace
Yes
Comparison of total RNA
isolated from different sources by AquaRNA. Bacterial culture
(0.5 ml), human cultured cells (500,000 cells), and rat liver
tissue (20 mg) were processed using the AquaRNA Kit.
Final DNA/RNA pellet was suspended in 100
ul DEPC water. Five-microliter of each prep
treated with or without DNase I was run in a non-denaturing
0.8% native agarose gel, showing the DNA, 28S (23S), 18S (16S), and 5S
RNA bands.
RNA integrity analysis.
Total RNA was extracted by AquaRNA and RNA extraction kit of
Competitor Q from a E. coli bacterial culture and the RNA integrity was
measured with Agilent 2100 Bioanalyzer.
Brief Protocol
1. Harvest the Cells
Pellet
~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at
14,000 xg for 60 sec. Aspirate or decant to discard the supernatant.
2. Extract the DNA/RNA
Add
100 ul AquaRNA solution (30 ml
in the kit for 300 minipreps) to the cell pellet. Suspend and lyse the cells
by vortex vigorously for 60 sec.
3. Remove the Debris
Centrifuge
the sample at 14,000 xg for 5 min to pellet the debris (Step 3 is optional
for cultured cells as all the cells are completely lysed and isopropanol may
be added directly to the cell lysate to pellet the DNA/RNA in Step 4).
4. Pellet the DNA/RNA
Transfer
the supernatant (~90 ul) to a new 0.5-ml
microfuge tube. Add 0.8 vol (~72 ul) of isopropanol and
vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA.
Decant to discard the supernatant (Note: Proteins remain in the isopropanol
supernatant and can be recovered by precipitation in 4 vol of acetone.) and
rinse the DNA/RNA pellet with 70% ethanol twice. Suspend the DNA/RNA pellet
in 100 ul of TE buffer or
deionized water. Centrifuge again to pellet any insoluble material and
transfer the DNA/RNA solution to a new tube. Remove DNA in the DNA/RNA prep
by DNase I digestion.
Ordering Information
Product
Name: AquaRNATM Kit
Product
Number: 5001, 5030
Application:
Isolation of DNA/RNA/proteins.
For in vitro research use only.
Size:
The kit is sufficient for the preparation of
5001: 10 minipreps (use 100 ul AquaRNA for a million culture cells)
5030: 300 minipreps
Kit
Contents: The AquaRNA Kit includes the
following items
5001: 1 ml AquaRNA
Solution, Instruction Manual
5030: 30 ml AquaRNA
Solution, Instruction Manual
Price:
5001: $10 each
5030: $199 each
Ordering:
To order, please
click the "BUY" button below.
