AquaRNATMis
a multifunctional aqueous solution for DNA, RNA, and protein
extraction. It functions to lyse the cells, inactivate RNases, and
extract DNA, RNA, and proteins. DNA and RNA are readily recovered
from the lysate by isopropanol precipitation, while proteins remain
in the supernatant and can be recovered by acetone precipitation. It
allows you to isolate DNA, RNA, and proteins for analyses from the
same cells, tissue, or organism. Additionally, AquaRNATM can be used to inactivate
and remove contaminating RNases in purified RNA samples to
ensure RNA integrity and stability.
AquaRNA
will change the way you think and work with RNA by simply
inactivating and removing endogenous contaminating RNases in your
purified RNA samples.
"RNA
is very unstable, labile, and must be stored at -80 °C."
- This is a misconception. If endogenous contaminating RNases are
removed, RNA samples can be stored at 4 °C
or even room temperatures for months without degradation.
"We
buy and use only RNase-free consumables." - This is a waste of
money. If RNA is not contaminated with endogenous RNases, your
chance of getting RNA degradation by using regular reagents,
solutions, tubes, and tips is very slim.
"RNA
work must be done at designated RNA-only workbench and use
RNA-only equipment." - This is again a waste of resources. Just
don't let your fingers, with or without gloves, touch the
inside of your RNA tube and its lid, you will be
fine.
"I am
always anxious
until I see the results of my RNA
experiments." - If the RNA is not
contaminated with residual RNases, you will find that RNA is no longer
difficult to work with and you will get consistent and
reproducible results.
RNase
Decontamination
AquaRNA can be used to treat purified RNA
that is contaminated with RNases. It can also be used
to treat plasmid DNA or in vitro transcribed RNA to remove
any contaminating RNases to ensure successful in vitro
transcription and translation reactions.
Not only for
RNA
AquaRNA can extract DNA, RNA, and
proteins from the same biological samples. If you are comparing two
genomic profiles, such as normal vs tumor tissues, why not compare
their RNA expression profiles, and further, their protein expression
profiles as well? In addition to maximizing the value of your
invaluable samples, you might uncover new correlations
and new differences.
Simple
Due to the
multifunctionality of the AquaRNA solution, its protocol is very
simple. After cell lysis, DNA/RNA are precipitated with isopropanol
leaving proteins in the supernatant.
Easy
No columns, no
resins, no slurries, no beads, no filters, no membranes, no
needles, no cartridges, no manifolds, no HPLC,
no ultracentrifuge.
No Nasty
Organic Solvents
AquaRNA extracts
DNA/RNA/proteins much like the famous Trizol®
(Invitrogen), but is much simpler
and requires no phenol and no chloroform. Yes,
it contains guanidine thiocyanate.
Scalable
One single AquaRNA
Kit does all: mini, midi, maxi, and HTP; no need to purchase
separate mini, midi, maxi, and
HTP kits.
High
Yield
Up to 100 ug of
DNA, 50 ug of RNA, and 2000 ug of proteins can be isolated
from 5
million mammalian cells or 50 mg animal tissue. AquaRNA
is especially effective for RNA extraction from plant
tissues.
Economic
AquaRNA kit is
priced at $199/kit for 300 minipreps, that is $0.66/miniprep or
approximately 1/6 the price of other RNA isolation kits. Each
miniprep uses 100 ul AquaRNA to extract DNA/RNA/proteins from 1
million cells.
Comparison
Competitor Q
AquaRNA
Isolation
Mechanism
Selective Binding
Selective Extraction
Isolation Format
Solid
Support
Aqueous
Solution
Price ($/miniprep)
3.84
0.66
Mercaptoethanol
Yes
No
Scalable
No
Yes
# of Solutions in the
Kit
4
1
Hands-on Time
(min)
30
30
Yield (ug RNA/mil
cells)
10-20
10-20
RNase
Contamination
?
No
Recover Small RNAs
No
Yes
Co-Purify
DNA/Proteins
Trace
Yes
Comparison of total RNA isolated from different
sources by AquaRNA. Bacterial culture (0.5 ml), human cultured
cells (500,000 cells), and rat liver tissue (20 mg) were
processed using the AquaRNA Kit. Final DNA/RNA pellet was
suspended in 100 ul DEPC water. Five-microliter of
each prep treated with or without DNase I was run in
a non-denaturing 0.5% native agarose gel, showing the DNA, 28S
(23S), 18S (16S), and 5S RNA bands.
Ordering
Information
Product
Name: AquaRNATM Kit
Product
Number: 5001, 5030
Application:
Isolation of
DNA/RNA/proteins. For in vitro research use
only.
Size: The kit is sufficient
for the preparation of
5001: 1ml for
10 minipreps
5030: 30 ml for
300 minipreps
Kit
Contents: The AquaRNA Kit includes the following
items
5001: 1
ml AquaRNA Solution, Instruction Manual
5030: 30
ml AquaRNA Solution, Instruction
Manual
Price:
5001: $10 each
5030: $199 each
Ordering:
To order, please click the "BUY"
button below.
