AquaRNATMis
a multifunctional aqueous solution for DNA, RNA, and protein
extraction. It functions to lyse the cells, inactivate RNases, and
extract DNA, RNA, and proteins simultaneously. DNA
and RNA are recovered from the cell lysate by isopropanol
precipitation, while proteins are recovered from the cell lysate by
acetone precipitation. AquaRNAÔ
enables concurrent isolation of DNA, RNA, and proteins from the same
specimen for analyses without using different DNA, RNA, and protein
extraction kits. Additionally, AquaRNATM can be used to inactivate
and remove contaminating RNases in purified RNA samples to
ensure RNA integrity and stability.
AquaRNA
will change the way you think and work with RNA by simply
inactivating and removing endogenous contaminating RNases in your
purified RNA samples.
"RNA
is very unstable and must be stored at -80 °C."
- Not true, if endogenous contaminating RNases are removed, RNA
samples can be stored at 4 °C
without degradation.
"RNA
work must be done at designated RNA-only workbench, use RNA-only
equipment and RNase-free consumables." - This is unnecessary, just
don't let your fingers, with or without gloves, touch the
inside of your RNA tube, your chance of getting RNA degradation by
using regular reagents, solutions, tubes, and tips is slim to
none.
"I am
always anxious
until I see the results of my RNA
experiments." - If the RNA is not
contaminated with residual RNases, you will find that RNA is no longer
difficult to work with and you will get consistent and
reproducible results every time.
RNase
Decontamination
AquaRNA can be used to treat purified RNA
that is contaminated with RNases. It can also be used
to treat plasmid DNA or in vitro transcribed RNA to remove
any contaminating RNases to ensure successful in vitro
transcription and translation.
Not only for
RNA
AquaRNA can extract DNA, RNA, and
proteins from the same biological samples. In addition to maximizing
the value of your invaluable biospecimens, you
might uncover new correlations and new differences by
analyzing DNA, RNA, and proteins at the same
time.
Simple
Due to the
multifunctionality of the AquaRNA solution, its protocol is very
simple. After cell lysis, DNA/RNA are precipitated with isopropanol
and proteins are precepitated with acetone. No columns, no resins,
no cartridges, and no ultracentrifuge are
needed.
No phenol
and chloroform
AquaRNA extracts
DNA/RNA/proteins much like the famous Trizol®
(Invitrogen), but it doesn't use phenol
and chloroform.
Scalable
One single AquaRNA
Kit does all: mini, midi, maxi, and HTP; no need to purchase
separate mini, midi, maxi, and
HTP kits.
High
Yield
Up to 50 ug of
DNA, 25 ug of RNA, and 1500 ug of proteins can be isolated
from 2.5
million mammalian cells or 25 mg animal tissue.
Furthermore, you will no longer have the problem of
getting the protein pellet into solution as you encounter with
Trizol.
Comparison
Competitor Q
AquaRNA
Isolation
Mechanism
Selective Binding
Selective Extraction
Isolation Format
Solid
Support
Aqueous
Solution
Price ($/miniprep)
3.84
0.66
Mercaptoethanol
Yes
No
Scalable
No
Yes
# of Solutions in the
Kit
4
1
Hands-on Time
(min)
30
30
Yield (ug RNA/mil
cells)
10-20
10-20
RNase
Contamination
?
No
Recover Small RNAs
No
Yes
Co-Purify
DNA/Proteins
Trace
Yes
Comparison of total RNA isolated from different
sources by AquaRNA. Bacterial culture (0.5 ml), human cultured
cells (500,000 cells), and rat liver tissue (20 mg) were
processed using the AquaRNA Kit. Final DNA/RNA pellet was
suspended in 100 ul DEPC water. Five-microliter of
each prep treated with or without DNase I was run in
a non-denaturing 0.5% native agarose gel, showing the DNA, 28S
(23S), 18S (16S), and 5S RNA bands.
Ordering
Information
Product
Name: AquaRNATM Kit
Product
Number: 5001, 5030
Application:
Isolation of
DNA/RNA/proteins. For in vitro research use
only.
Size: The kit is sufficient
for the preparation of
5001: 1ml for
10 minipreps
5030: 30 ml for
300 minipreps
Kit
Contents: The AquaRNA Kit includes the following
items
5001: 1
ml AquaRNA Solution, Instruction Manual
5030: 30
ml AquaRNA Solution, Instruction
Manual
Price:
5001: $10 each
5030: $199 each
Ordering:
To order, please click the "BUY"
button below.
