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AquaRNATM is
a multifunctional aqueous solution for DNA, RNA, and protein
extraction. It functions to lyse the cells, inactivate RNases, and
extract DNA, RNA, and proteins. DNA and RNA are readily recovered
from the lysate by isopropanol precipitation, while proteins remain
in the supernatant and can be recovered by acetone precipitation. It
allows you to simultaneously isolate DNA, RNA, and proteins for
analyses from the same cells, tissue, or organism, making
it possible to get more out of limited and
precious samples, or to save time, money and labor by
doing three extractions at the same time.
Features
Innovative
One-of-a-kind aqueous solution
that irreversibly inactivates RNases. If
you have experienced the degradation of purified RNA
during its storage or analysis and you know that SDS, guanidine
thiocyanate, phenol/chloroform, and even autoclaving could not
irreversibly inactivate RNases, you would appreciate this innovation
and what it means to your research.
Not only for
RNA
AquaRNA can simultaneously extract DNA,
RNA, and proteins from the same biological samples. If you are
comparing two genomic profiles, such as normal vs tumor tissues, why
not compare their RNA expression profiles, and further, their
protein expression profiles as well? In addition to maximizing the
value of your invaluable samples, you might uncover new
correlations and new differences.
Simple
Due to the
multifunctionality of the AquaRNA solution, its protocol is very
simple. After cell lysis, DNA/RNA are precipitated with isopropanol
leaving proteins in the supernatant.
Easy
No columns, no
resins, no slurries, no beads, no filters, no membranes, no
needles, no cartridges, no manifolds, no HPLC,
no ultracentrifuge.
No Nasty
Organic Solvents
No phenol, no
chloroform, no saturated butanol, no ethidium bromide, no cesium
chloride, no 2-mercaptoethanol. Warning: Yes, it contains
guanidine thiocyanate.
Pure
The purified RNA
is no longer "very unstable" or tricky to work with,
because it is no longer contaminated with endogenous
RNases. Your RNA's integrity maintains during storage and
subsequent analysis.
Scalable
One single AquaRNA
Kit does all: mini, midi, maxi, and HTP; no need to purchase
separate mini, midi, maxi, and
HTP kits.
High
Yield
Up to 100 ug of
DNA, 50 ug of RNA, and 2000 ug of proteins can be isolated
from 5
million mammalian cells or 50 mg animal
tissue.
Economic
AquaRNA kit is
priced at $199/kit for 300 minipreps, that is $0.66/miniprep or
approximately 1/6 the price of other RNA isolation kits. Each
miniprep uses 100 ul AquaRNA to extract DNA/RNA/proteins from 1
million cells.
Comparison
| |
Competitor Q |
AquaRNA |
|
Isolation
Mechanism |
Selective Binding |
Selective Extraction |
|
Isolation Format |
Solid
Support |
Aqueous
Solution |
|
Price ($/miniprep) |
3.84 |
0.66 |
|
Mercaptoethanol |
Yes |
No |
|
Scalable |
No |
Yes |
|
# of Solutions in the
Kit |
4 |
1 |
|
Hands-on Time (min) |
30 |
30 |
|
Yield (ug RNA/mil cells) |
10-20 |
10-20 |
|
RNase
Contamination |
? |
No |
|
Recover Small RNAs
|
No |
Yes |
|
Co-Purify
DNA/Proteins |
Trace |
Yes |
Comparison of total RNA isolated from different
sources by AquaRNA. Bacterial culture (0.5 ml), human cultured
cells (500,000 cells), and rat liver tissue (20 mg) were
processed using the AquaRNA Kit. Final DNA/RNA pellet was
suspended in 100 ul DEPC water. Five-microliter of
each prep treated with or without DNase I was run in
a non-denaturing 0.5% native agarose gel, showing the DNA, 28S
(23S), 18S (16S), and 5S RNA bands.
Ordering
Information
Product
Name: AquaRNATM
Kit
Product
Number: 5001, 5030
Application:
Isolation of
DNA/RNA/proteins. For in vitro research use
only.
Size: The kit is sufficient
for the preparation of
- 5001: 1ml for
10 minipreps
- 5030: 30 ml for
300 minipreps
Kit
Contents: The AquaRNA Kit includes the following
items
- 5001:
1
ml AquaRNA Solution, Instruction Manual
- 5030:
30
ml AquaRNA Solution, Instruction
Manual
Price:
- 5001:
$10 each
- 5030:
$199 each
Ordering:
To order, please click the "BUY"
button below.
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