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AQUARNA


AquaRNA
TM is a multifunctional aqueous solution-based reagent for DNA, RNA, and protein extraction. This single solution will lyse the cells, inactivate degradative enzymes, and extract DNA, RNA, and proteins. DNA and RNA are recovered from the cell lysate by isopropanol precipitation, while proteins remain soluble in the isopropanol solution and can be recovered by acetone precipitation. AquaRNA enables concurrent isolation of DNA, RNA, and proteins from the same specimen without using different DNA, RNA, and protein extraction kits.


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Features 


RNA myths

AquaRNA will change the way you think and work with RNA by simply inactivating and removing endogenous contaminating RNases in your RNA samples. 

  • "RNA work must be done at designated RNA-only workbench, use RNA-only equipment and RNase-free consumables." - Unnecessary. If you won't touch the inside of your RNA tube, your chance of getting RNA degradation by using regular reagents and tubes is slim to none. 
  • "I am always anxious until I see the results of my RNA experiments." - If the RNA sample is not contaminated with RNases, you will get consistent and reproducible results every time.

RNase Decontamination

 

AquaRNA may be used to remove contaminating RNases from purified RNA or remove contaminating RNases in purified plasmid DNA for in vitro transcription.

 

Not only for RNA

 

AquaRNA can extract DNA, RNA, and proteins from the same biological samples concurrently and allow you to maximize the value of your biospecimens. 

Simple

Due to the multifunctionality of the AquaRNA solution, its protocol is very simple. After cell lysis, DNA/RNA are precipitated with isopropanol and proteins are precipitated with acetone. No columns are needed.

No phenol and chloroform

AquaRNA extracts DNA/RNA/proteins without using phenol and chloroform. You will not have  problem solubilizing the protein pellet as you often encounter using organic extraction.

Scalable

One AquaRNA Kit does all. No need to purchase separate mini, midi, maxi, and HTP kits.



Comparison


 

 Competitor 

 AquaRNA

Isolation Format

Solid support

Aqueous solution

Price ($/mini)

3.84

0.66

Mercaptoethanol

Yes

 No

Scalable

No

Yes

# of Solutions 

4

1

Small RNAs

Not recovered

Recovered

DNA

Not recovered

Recovered



bioanalyzer.jpg

Total RNA was extracted from an E. coli culture and the RNA integrity was measured with Agilent 2100 Bioanalyzer.

rna gel for web3.jpg

DNA/RNA was extracted from bacterial culture, mammalian culture, and rat liver tissue with AquaRNA, and treated with or without DNase I. Shown are the DNA, 28S (23S), 18S (16S), and 5S RNA bands.



Brief Protocol 

(Please refer to the Instruction Manual for DNA/RNA/protein extraction from bacteria, cultured cells, animal tissues, and plant tissues, etc.)


1. Harvest the Cells

Pellet ~0.5-2 million cultured cells in a 1.5-ml microfuge tube by centrifugation at 14,000 xg for 60 sec. Aspirate or decant to discard the supernatant. (Note: Centrifuging to pellet the cells may not be needed as long as 1 vol of AquaRNA is mixed with 1 vol of cell suspension for DNA/RNA extraction.)

2. Extract the DNA/RNA

Add 100 ul AquaRNA solution (30 ml in the kit for 300 minipreps) to the cell pellet (or equal volume of cell suspension). Vortex vigorously for 60 sec to lyse the cells.

3. Pellet the DNA/RNA

Add 0.8 vol (~100 ul) of isopropanol to the crude lysate and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA. Decant to discard the supernatant (Note: Proteins remain in the isopropanol supernatant and can be recovered by precipitation in 4 vol of acetone.) and rinse the DNA/RNA pellet with 70% ethanol twice. Suspend the DNA/RNA pellet in 100 ul of TE buffer or deionized water. Centrifuge again to pellet any insoluble material and transfer the DNA/RNA solution to a new tube. Remove DNA in the DNA/RNA prep by DNase I digestion.






Ordering Information

Product Name:    AquaRNATM Kit

Product Number:   5001, 5030

Application:    Isolation of DNA/RNA/proteins. For in vitro research use only.

Size:    The kit is sufficient for the preparation of 

      • 5001: 10 minipreps (use 100 ul AquaRNA per million culture cells)
      • 5030: 300 minipreps 

Kit Contents:   The AquaRNA Kit includes the following items

      • 5001: 1 ml AquaRNA Solution, Instruction Manual
      • 5030: 30 ml AquaRNA Solution, Instruction Manual

Price:  

      • 5001: $10 each
      • 5030: $199 each

Ordering:    To order, please click the "BUY" button below.

  

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Copyright  2014
MultiTarget Pharmaceuticals, LLC - All rights reserved