fecal DNA,RNA extraction,biospecimen,DNA extraction,protein extraction,preservative


 
   AquaPlasmid, AquaGenomic, & AquaRNA       aqueous solutions for nucleic acid isolation and purification

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AQUARNA

 

AquaRNATM is a multifunctional aqueous solution for DNA, RNA, and protein extraction. It functions to lyse the cells, inactivate RNases, and extract DNA, RNA, and proteins. DNA and RNA are readily recovered from the lysate by isopropanol precipitation, while proteins remain in the supernatant and can be recovered by acetone precipitation. It allows you to isolate DNA, RNA, and proteins for analyses from the same cells, tissue, or organism. Additionally, AquaRNATM can be used to inactivate and remove contaminating RNases in purified RNA samples to ensure RNA integrity and stability.

 

 


  Features

 

Concept-Changing

AquaRNA will change the way you think and work with RNA by simply inactivating and removing endogenous contaminating RNases in your purified RNA samples.

  • "RNA is very unstable, labile, and must be stored at -80 °C." - This is a misconception. If endogenous contaminating RNases are removed, RNA samples can be stored at 4 °C or even room temperatures for months without degradation.
  • "We buy and use only RNase-free consumables." - This is a waste of money. If RNA is not contaminated with endogenous RNases, your chance of getting RNA degradation by using regular reagents, solutions, tubes, and tips is very slim.
  • "RNA work must be done at designated RNA-only workbench and use RNA-only equipment." - This is again a waste of resources. Just don't let your fingers, with or without gloves, touch the inside of your RNA tube and its lid, you will be fine.
  • "I am always anxious until I see the results of my RNA experiments." - If the RNA is not contaminated with residual RNases, you will find that RNA is no longer difficult to work with and you will get consistent and reproducible results.

RNase Decontamination

 

AquaRNA can be used to treat purified RNA that is contaminated with RNases. It can also be used to treat plasmid DNA or in vitro transcribed RNA to remove any contaminating RNases to ensure successful in vitro transcription and translation reactions.

 

Not only for RNA

 

AquaRNA can extract DNA, RNA, and proteins from the same biological samples. If you are comparing two genomic profiles, such as normal vs tumor tissues, why not compare their RNA expression profiles, and further, their protein expression profiles as well? In addition to maximizing the value of your invaluable samples, you might uncover new correlations and new differences.

 

Simple

Due to the multifunctionality of the AquaRNA solution, its protocol is very simple. After cell lysis, DNA/RNA are precipitated with isopropanol leaving proteins in the supernatant. 

Easy

No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no HPLC, no ultracentrifuge.

No Nasty Organic Solvents

AquaRNA extracts DNA/RNA/proteins much like the famous Trizol® (Invitrogen), but is much simpler and requires no phenol and no chloroform. Yes, it contains guanidine thiocyanate.

Scalable

One single AquaRNA Kit does all: mini, midi, maxi, and HTP; no need to purchase separate mini, midi, maxi, and HTP kits.

High Yield

Up to 100 ug of DNA, 50 ug of RNA, and 2000 ug of proteins can be isolated from 5 million mammalian cells or 50 mg animal tissue. AquaRNA is especially effective for RNA extraction from plant tissues.

Economic

AquaRNA kit is priced at $199/kit for 300 minipreps, that is $0.66/miniprep or approximately 1/6 the price of other RNA isolation kits. Each miniprep uses 100 ul AquaRNA to extract DNA/RNA/proteins from 1 million cells.

 


Comparison

 

 

 Competitor Q

 AquaRNA

Isolation Mechanism

Selective Binding

Selective Extraction

Isolation Format

Solid Support

Aqueous Solution

Price ($/miniprep)

3.84

0.66

Mercaptoethanol

Yes

 No

Scalable

No

Yes

# of Solutions in the Kit

4

1

Hands-on Time (min)

30

30

Yield (ug RNA/mil cells)

10-20

10-20

RNase Contamination

 ?

No

Recover Small RNAs

ar vs q.jpg

No

Yes

Co-Purify DNA/Proteins

Trace

Yes

 

rna gel for web3.jpg

Comparison of total RNA isolated from different sources by AquaRNA. Bacterial culture (0.5 ml), human cultured cells (500,000 cells), and rat liver tissue (20 mg) were processed using the AquaRNA Kit. Final DNA/RNA pellet was suspended in 100 ul DEPC water. Five-microliter of each prep treated with or without DNase I was run in a non-denaturing 0.5% native agarose gel, showing the DNA, 28S (23S), 18S (16S), and 5S RNA bands.

bioanalyzer.jpg


Ordering Information

Product Name:    AquaRNATM Kit

Product Number:   5001, 5030

Application:    Isolation of DNA/RNA/proteins. For in vitro research use only.

Size:    The kit is sufficient for the preparation of

      • 5001: 1ml for 10 minipreps
      • 5030: 30 ml for 300 minipreps

Kit Contents:   The AquaRNA Kit includes the following items

      • 5001: 1 ml AquaRNA Solution, Instruction Manual
      • 5030: 30 ml AquaRNA Solution, Instruction Manual

Price:  

      • 5001: $10 each
      • 5030: $199 each

Ordering:    To order, please click the "BUY" button below.  

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