Stool is an accessible and noninvasive source of biospecimen. Fecal DNA/RNA originated from the host, intestinal bacteria, viruses, fungi, or parasites, and incompletely digested foods are molecular fingerprints of the host and its health. AquaStool is a multifunctional aqueous solution-based reagent for fecal sample stabilization, DNA and RNA extraction and PCR inhibitor removal. It may be used to extract fecal DNA for non-invasive genotyping of transgenic animals. AquaStool may be used to preserve and extract DNA/RNA from human stools for host and gut microbiota research. AquaStool may also be used to extract DNA from tree tissues, which are particularly challenging with existing DNA extraction methods.
AquaStool combines the functions of specimen stabilization, DNA/RNA extraction, and PCR inhibitor removal.
AquaStool can be used to extract fecal DNA and enables noninvasive genotyping of transgenic animals and monitoring of wildlife animals.
Recover host and microbial DNA and RNA in feces
AquaStool extracts fecal DNA and RNA originated from the host, commensal bacteria, invasive microbes, and incompletely digested foods, enabling the study of gut microbiome; diagnosis of microbial infections.
Streamline fecal sample collection and DNA/RNA extraction
AquaStool may be used to collect fecal specimens in the field, stabilize the DNA, ship the specimens at ambient temperature and extract the DNA without using other extraction kits.
Comparison of tail and fecal DNA extractions with our AquaGenomic and AquaStool reagents
(Please refer to the Instruction Manual for mouse fecal and wood DNA extraction protocols)
1. Extract the DNA/RNA
Add 50 mg of human fecal sample to a 1.5-ml microfuge tube preloaded with 0.5 ml AquaStool solution and ~100 ug of sand. Vortex the tube upside down at top speed for a few minutes (use a multichannel bead beater, if available). Centrifuge at 14,000 xg for 5 min to pellet the fecal debris. Optional: Add 250 ul of AquaRemove (#1208, order separately and dilute it with an equal volume of isopropanol before use) to the clear lysate. Vortex to mix well and centrifuge at 14,000 xg again for 5 min to pellet the debris.
2. Pellet the DNA/RNA
Transfer 0.6 ml cleared lysate to a 1.5-ml tube and add 0.7 volume (0.42 ml) isopropanol. Vortex and centrifuge at 14,000 xg for 5 min to pellet the DNA/RNA. Decant to discard the supernatant. Rinse the pellet by filling up the tube with 70% ethanol from a squirt bottle (be sure to rinse the entire interior of the tube, including the cap and the mouth of the tube), and decant to discard the ethanol solution. Repeat the ethanol rinse once. Tap the tube on a clean paper towel to remove residual ethanol and air-dry the pellet for 5-10 min.
3. Solubilize the DNA/RNA
Add 0.4 ml deionized water to the pellet, Pipet and vortex to fully disperse the pellet. Incubate at 22 °C for 15 min and then centrifuge at 14,000 xg for 5 min to pellet any insoluble. Transfer the clear DNA/RNA solution to a new tube and store at –20 or -80 °C (Note: Centrifuge the frozen DNA solution to remove any insoluble before its use in a PCR reaction).
Product Name: AquaStoolTM Kit
Product Number: 7001, 7030
Application: DNA/RNA extraction from fecal, soil and wood samples. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaStool Kit includes the following items
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