AquaStoolTM is a
multifunctional aqueous solution-based reagent for fecal DNA and RNA
preservation and extraction, and fecal PCR inhibitor removal. It may be
used to extract
fecal DNA for non-invasive genotyping of transgenic animals. Unlike
tail/toe/ear snipping, fecal sampling is not limited by animal age,
physiopathological condition, and sampling frequency. Fecal DNA is
particularly useful for verifying adult animals’ genotype and
may also be used to stabilize and extract fecal DNA/RNA from human
stools for host and microbiome studies.
AquaStool combines the functions of specimen stabilization, DNA extraction, and PCR inhibitor removal in a single solution.
AquaStool can be used to extract fecal DNA and enables noninvasive genotyping of transgenic animals and monitoring of wildlife animals.
Recover DNA from the host and microbes in the stool
AquaStool extracts fecal DNA and RNA originated from the host, commensal bacteria, invasive viruses, fungi, or parasites, and incompletely digested foods, enabling the study of human and animal microbiome; diagnosis of bacterial, viral, fungal, and parasitic infections.
Not only for extraction but also for specimen stabilization
AquaStool may be used to collect fecal specimens in the field. It will stabilize the DNA and allow shipping the fecal specimens to the laboratory at ambient temperature.
We offer AquaStool for fecal DNA extraction and AquaGenomic for tail DNA extraction. Here is a comparison of the two methods.
Comparison of tail DNA and fecal DNA extraction
* AquaGenomic (#2030) may also be used to extract fecal DNA from mice (not yet tested on other species). AquaGenomic is nontoxic and its lysate may be used directly for PCR.
(Please refer to the Instruction Manual for fecal DNA/RNA extraction from human stool specimens.)
AquaStool mouse fecal DNA extraction protocol
1. Collect the fecal sample
Transfer a mouse to a cage floored with a clean paper towel. Scoop up a fecal pellet with a microfuge tube labeled with the animal ID (fecal pellets may be stored at RT for months after being air-dried by incubating in open tubes on a 37 °C heat bloc for 24 hrs).
2. Extract the DNA
Add 150 ul AquaStool (30 ml in the kit for 200 extractions) to each fecal pellet. Soak the pellet until it becomes soften and vortex vigorously to homogenize the fecal material (if needed, use a pipette tip to break up the fecal pellet). For microbial DNA extraction, vortex the fecal material in the presence of white sand or homogenize it with a bead beater.
3. Remove the Debris
Centrifuge the sample at 14,000 xg for 10 min to pellet the debris.
4. Pellet the DNATransfer the supernatant (~90 ul) to a new 0.5-ml microfuge tube. Add 0.8 vol (~72 ul) of isopropanol and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant and rinse the DNA pellet with 70% ethanol twice. Suspend the DNA pellet in 100 ul of TE buffer or deionized water. Centrifuge again to pellet any insoluble material and transfer the DNA solution to a new tube (Note: It is critical to centrifuge the DNA solution again before adding it to a PCR reaction, as some insoluble material, which contain PCR inhibitors, may develop during storage.).
AquaGenomic mouse fecal DNA extraction protocol
Product Name: AquaStool Kit
Product Number: 7001, 7030
Application: DNA/RNA extraction from fecal specimens. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaStool Kit includes the following items
Ordering: To order, please click the "BUY" button below.
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