AquaStoolTMis
a
multifunctional aqueous solution for DNA and RNA isolation and
purification from fecal biospecimens. This single solution functions
to stabilize, preserve, extract fecal DNA/RNA, and remove
contaminating PCR and RT-PCR inhibitors abundant in these
biospecimens. It streamlines stool collection, stabilization,
preservation, DNA/RNA extraction, and molecular analysis.
Approximately 150-200 ug
total nucleic acids can be isolated from 50 mg of human
feces.
Stool
is a readily accessible, abundant, under-utilized, and noninvasive
source of biospecimen. It contains trillions of microorganisms
living in our aerodigestive and gastrointestinal tracks, millions of
human cells sloughed from these organs every day, and numerous
macrophages and lymphocytes migrating between our gut lumen and
blood circulation. It can be an unmatched and excellent barometer of
our health and diseases, in particular, gastrointestinal cancers,
infections, inflammations, and microbiome. However, stool is one of
the most difficult and challenging biospecimens to obtain
high-quality DNA and RNA for molecular analysis due to its
containing of large amount of digestive enzymes that destroy the DNA
and RNA prior to and during their extraction, and numerous DNA and
RNA contaminants that interfere subsequent molecular
analysis. AquaStool technology will facilitate
the growth of stool-based sciences and accelerate the drive toward
personalized medicine.
Multifunctionalities
AquaStool
combines the functions of biospecimen stabilization, preservation,
DNA/RNA extraction, and PCR inhibitor removal in a single solution.
It streamlines biospecimen preservation, DNA/RNA extraction, and
molecular analysis. It makes fecal DNA/RNA extraction simple, fast,
economic, and convenient.
Extract both Fecal DNA and
RNA
AquaStool extracts both fecal DNA and RNA
from the same fecal specimen (Fig.),
enabling noninvasive genotyping and RNAtyping for the
identification of transgenic animals; monitoring wildlife animals;
diagnosis of gastrointestinal disorders, and the study of RNA
expression and its relationship to genetic
variation.
Extract DNA and RNA of the Host
and Microbes in the Feces
AquaStool extracts total nucleic acids in
feces, including DNA and RNA from the host, commensal
bacteria, any invasive viruses, fungi, or parasites, and
incompletely digested foods (Fig.), enabling forensic
identifications; the study of human and animal microbiome; and
diagnosis of bacterial, viral, fungal, and parasitic infections.
Not only for Fecal
Specimens
The
challenges we face in DNA and RNA extraction from
fecal specimens exceed the challenges we will encounter in most
other biospecimens. Therefore, AquaStool protocols can be
readily modified and adapted to DNA and RNA preservation and
extraction from other biomaterials, such as microbes,
culture cells, animal and plant tissues, and even insects, making
AquaStool a universal reagent for the study and correlation of
genetic mutation and gene expression of any life
form.
Comparison
Comparison
of Techniques for Biospecimen
Stabilization
Formalin
R*later
AquaStool
Mechanism of Stabilization
Cross-linking
High
Salt Dehydration
Enzyme
Inactivation
Enzymatic
Activity
Irreversible
Reversible
Irreversible
Ethanol
Precipitation
N/A
Incompatible
Compatible
Damage
to Specimen
Yes
No
No
Inactivation
of Pathogens
Yes
No
Yes
PCR
Inhibitor Removal
No
No
Yes
Stabilization
and Extraction
No
No
Yes
Ordering
Information
Product
Name: AquaStool Kit
Product
Number: 7001, 7030
Application:DNA/RNA extraction from fecal &
other specimens. For in vitro research use only.
Size: The
kit is sufficient for the preparation of
7001: 1ml for 2
extractions
7030: 30 ml for 60
extractions
Kit Contents: The
AquaStool Kit includes the following
items
7001: 1
ml AquaStool Solution, Instruction Manual
7030: 30
ml AquaStool Solution, Instruction
Manual
Price:
7001: $10 each
7030: $199 each
Ordering:
To order, please
click the "BUY"
button below.
