Stool is an accessible and underutilized noninvasive source of biospecimen. Fecal DNA/RNA originated from the host, intestinal bacteria, viruses, fungi, or parasites, and incompletely digested foods are molecular fingerprints of the host and its health. AquaStoolTM is a multifunctional aqueous solution-based reagent for fecal sample stabilization, DNA and RNA extraction and PCR inhibitor removal. It may be used to extract fecal DNA for non-invasive genotyping of transgenic animals. Fecal sampling is not limited by animal age, physiopathological condition, or sampling frequency. AquaStool may be used to preserve and extract DNA/RNA from human stools for host and gut microbiota research. AquaStool may also be used to extract DNA from tree tissues, which is particularly challenging with existing DNA extraction methods..
AquaStool combines the functions of specimen stabilization, DNA/RNA extraction, and PCR inhibitor removal.
AquaStool can be used to extract fecal DNA and enables noninvasive genotyping of transgenic animals and monitoring of wildlife animals.
Recover host and microbial DNA and RNA in feces
AquaStool extracts fecal DNA and RNA originated from the host, commensal bacteria, invasive viruses, fungi, or parasites, and incompletely digested foods, enabling the study of human and animal microbiome; diagnosis of bacterial, viral, fungal, and parasitic infections.
The powerful combination of AquaStool, sand and sonication lysis can produce 100-200 ug of DNA from 50 mg of feces. The sheared DNA is ready for next generation sequencing and could reveal a different but "true" microbiota profile of your samples.
Streamline fecal sample collection and DNA/RNA extraction
AquaStool may be used to collect fecal specimens in the field. It will stabilize the DNA/RNA and allow the shipping of the fecal samples to the laboratory at ambient temperature, therefore, streamline fecal sample collection, transport and DNA/RNA extraction.
We offer AquaStool for fecal DNA extraction and AquaGenomic for tail DNA extraction. Below is a comparison of the two methods.
Comparison of tail DNA and fecal DNA extraction
(Please refer to the Instruction Manual for wood DNA extraction protocol)
AquaStool mouse fecal DNA extraction protocol
1. Collect the fecal sample
Transfer a mouse to a cage floored with a clean paper towel. Scoop up a fecal pellet with a microfuge tube labeled with the animal ID (fecal pellets may be stored at RT for months after being air-dried by incubating in open tubes on a 37 °C heat bloc for 24 hrs).
2. Extract the DNA
Add 150 ul AquaStool (30 ml in the kit for 200 extractions) to each fecal pellet. Soak the pellet until it becomes soften and vortex vigorously to homogenize the fecal material (if needed, use a pipette tip to break up the fecal pellet). For microbial DNA extraction, vortex the fecal material in the presence of ~ 50 ug of white sand or homogenize it with a bead beater. Centrifuge the sample at 14,000 xg for 10 min to pellet the debris.
3. Pellet the DNATransfer the supernatant (~90 ul) to a new 0.5-ml microfuge tube. Add 0.7 vol (~63 ul) of isopropanol and vortex to mix. Centrifuge at 14,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant. Rinse the pellet by filling up the tube with 70% ethanol from a squirt bottle (be sure to rinse the entire interior of the tube, including the cap and the mouth of the tube), and decant to discard the ethanol solution. Repeat the ethanol rinse once. Tap the tube on a clean paper towel to remove residual ethanol and air-dry the pellet for 5-10 min.
4. Solubilize the DNA
Add 100 ul deionized water to the pellet, Pipet and vortex to fully disperse the pellet. Incubate at 22 °C for 15 min and then centrifuge at 14,000 xg for 5 min to pellet any insoluble. Transfer the clear DNA solution to a new tube and store at –20 °C (Note: Centrifuge the frozen DNA solution to remove any insoluble before its use in a PCR reaction.).
AquaStool human fecal DNA/RNA extraction protocol
Product Name: AquaStool Kit
Product Number: 7001, 7030
Application: DNA/RNA extraction from fecal samples. For in vitro research use only.
Size: The kit is sufficient for the preparation of
Kit Contents: The AquaStool Kit includes the following items
Ordering: To order, please click the "BUY" button below.
Note: Order AquaRemove (# 1208, 8 ml for $49) separately for human fecal DNA/RNA purification. Dilute 8 ml AquaRemove with 8 ml isopropanol before use.
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