AquaStoolTMis
a
multifunctional aqueous solution-based reagent for fecal DNA and RNA
extraction and fecal PCR inhibitor removal. It may be used to extract
fecal DNA for non-invasive genotyping of transgenic animals. Unlike
tail/toe/ear snipping, fecal sampling is not limited by animal age,
physiopathological condition, and sampling frequency. It is
particularly useful for fecal DNA extraction to verify adult
animals’ genotype and transgene stability.
AquaStool
combines the functions of biospecimen stabilization, DNA/RNA
extraction, and PCR inhibitor removal in a single solution.
Non-invasive genotyping
AquaStool can be used to extract fecal DNA
and enables noninvasive genotyping of transgenic animals and monitoring of wildlife animals.
Recover DNA from the host
and microbes in the stool
AquaStool may be used to extract total nucleic acids in
feces, including DNA and RNA from the host, commensal
bacteria, any invasive viruses, fungi, or parasites, and
incompletely digested foods, enabling the study of human and animal microbiome; and
diagnosis of bacterial, viral, fungal, and parasitic infections.
Not only for fecal specimens
AquaStool can be used to extract DNA and RNA from other biospecimens, such as microbes,
culture cells, animal and plant tissues, and even insects.
Comparison
Comparison
of Techniques for Biospecimen
Stabilization
Formalin
R*later
AquaStool
Mechanism of Stabilization
Cross-linking
High
Salt Dehydration
Enzyme
Inactivation
Enzymatic
Activity
Irreversible
Reversible
Irreversible
Ethanol
Precipitation
N/A
Incompatible
Compatible
Damage
to Specimen
Yes
No
No
Inactivation
of Pathogens
Yes
No
Yes
PCR
Inhibitor Removal
No
No
Yes
Stabilization
and Extraction
No
No
Yes
Brief Protocol
1. Collect the fecal sample
Transfer
a mouse to a cage floored with a clean paper towel. Scoop up a fecal pellet
with a microfuge tube labeled with the animal ID (fecal pellets may be stored
at RT for months after being air-dried by incubating in open tubes on a 37 °C
heat bloc for 24 hrs).
2. Extract the DNA
Add
150 ul AquaStool solution (30
ml in the kit for 200 extractions) to each fecal pellet. Soak the pellet
until it becomes soften and vortex vigorously to homogenize the fecal
material (if needed, use a pipette tip to break up the fecal pellet). For
microbial DNA extraction, vortex the fecal material in the presence of white sand or
homogenize it with a bead beater or sonicator.
3. Remove the Debris
Centrifuge
the sample at 14,000 xg for 10 min to pellet the debris.
4. Pellet the DNA
Transfer the supernatant (~90 ul) to a new 0.5-ml
microfuge tube. Add 0.8 vol (~72 ul) of isopropanol and vortex to mix. Centrifuge at
14,000 xg for 5 min to pellet the DNA. Decant to discard the supernatant and
rinse the DNA pellet with 70% ethanol twice. Suspend the DNA pellet in 100 ul
of TE buffer or deionized water. Centrifuge again to pellet any insoluble
material and transfer the DNA solution to a new tube (Note: It is critical to
centrifuge the DNA solution again before adding it to a PCR reaction, as
some insoluble material, which contain PCR inhibitors, may develop during
storage.).
Ordering
Information
Product
Name: AquaStool Kit
Product
Number:7001, 7030
Application:DNA/RNA extraction from fecal specimens. For in vitro research use only.
Size: The
kit is sufficient for the preparation of
7001: 6
extractions (use 150 ul AquaStool for a mouse fecal pellet)
7030: 200
extractions
Kit Contents: The
AquaStool Kit includes the following
items
7001: 1
ml AquaStool Solution, Instruction Manual
7030: 30
ml AquaStool Solution, Instruction
Manual
Price:
7001: $10 each
7030: $199 each
Ordering:
To order, please
click the "BUY"
button below.
