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We offer several aqueous
solution based reagent products for research and education
applications. All of our products are inexpensive, scalable, and
simple to use. For a complete list of our reagent products,
including accessory products, please browse either the
PO Order page or
the Credit Card
Order page.
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AquaPlasmidTM -
For isolation
and purification of plasmid DNA.
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AquaGenomicTM - For
isolation
and purification of genomic DNA.
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AquaRNATM - For isolation
of DNA, RNA, and proteins.
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AquaBluerTM - For assessing
cell viability, cell proliferation, and
cytotoxicity.
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AquaStoolTM - For
fecal DNA/RNA preservation and extraction.
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AquaPrecipiTM - For purification of DNA free of
PCR inhibitors.
AquaPlasmidTM

AquaPlasmid is a
novel aqueous solution-based technology for the isolation and
purification of plasmid DNA. Its manufacturing cost is significantly
lower than spin column based kits and the saving is passed on to the
end users. AquaPlasmid protocol
is as simple as standard alkaline lysis. It is
scalable. Mini, midi, and maxiprep can be completed in as little as
30 minutes. The DNA yield can be up to 10 ug/ml
culture. The isolated DNA
is ultrapure and its A260/A280 ratio often achieves 2.0.
AquaPlasmid
offers
significant cost, time, and labor savings to its end users.
For more information and ordering, please
click .
AquaGenomicTM

AquaGenomicTM is a nontoxic aqueous solution
for genomic DNA isolation and purification from a variety of cells
and tissues. It is a multi-functional "magic" solution.
It can be used to suspend the cells, lyse the cells,
extract the DNA, and precipitate other cellular components in a
single step. AquaGenomicTM probably is the
simplest and most powerful genomic DNA extraction solution ever
developed. You simply add AquaGenomic Solution to the cell pellet,
vortex to extract the DNA, centrifuge to remove cell debris, and
then add isopropanol to precipitate pure genomic DNA. Mini, midi,
maxi, or even tissue prep can be completed in as little as 15
minutes. The yield is about 5-10 ug
DNA from 1-2 million cultured human cells, 200 ul of whole blood, 15
mg of feces, 1 ml of microbial cultures, 5-10 mg of animal tissues,
or 10-20 mg of plant tissues.
For more information and ordering, please click
.
AquaRNATM

AquaRNATM is
a multifunctional aqueous solution for DNA, RNA, and protein
extraction. It functions to lyse the cells, inactivate RNases, and
extract DNA, RNA, and proteins. DNA and RNA are readily recovered
from the lysate by isopropanol precipitation, while proteins remain
in the supernatant and can be recovered by acetone precipitation. It
allows you to simultaneously isolate DNA, RNA, and proteins for
analyses from the same cells, tissue, or organism, making
it possible to get more out of your limited and precious
samples, or
to save time, money and labor by doing three extractions
concurrently. Additionally,
AquaRNATM can be used to
inactivate residual RNases in purified RNA and DNA samples to
ensure their integrity and stability.
For more information and ordering, please click
.
AquaBluerTM

AquaBluerTM
is a specially formulated 10x concentrated and Bluer redox indicator
for assessing cell viability, cell proliferation, and cytotoxicity.
The fluorescence
intensity of AquaBluerTM
at 540ex/590em is proportional to the number of viable cells in the
sample. In addition to being nontoxic, simple to use, sensitive, and
reproducible, AquaBluerTM has several distinctive advantages
over comparable alamar blue and other similar reagents. First, it’s
10x concentrated and costs ~37% less. Secondly, it has 2x wider
linear assay range and could accurately measure near confluent
cultures where alamar blue fails. Finally, it’s more stable. It may
be stored in the dark at 4-22 °C up to 12 months.
For
more information and ordering, please click .
AquaStoolTM

Stool
is an accessible, abundant, under-utilized, and noninvasive source
of biospecimen. Fecal DNA and RNA originated from the host,
commensal bacteria, any viruses, fungi, or parasites, and
incompletely digested foods are molecular fingerprints of the host
and its health and disease. AquaStool
is a multifunctional aqueous solution for DNA and RNA isolation and
purification from fecal biospecimens. This single solution functions
to stabilize, preserve, extract fecal DNA/RNA, and remove
contaminating PCR and RT-PCR inhibitors abundant in these
biospecimens. It streamlines stool collection, stabilization,
preservation, DNA/RNA extraction, and molecular
analysis.
For
more information and ordering, please click .
AquaPrecipiTM

AquaPrecipiTM is a polymer solution for the removal
of PCR inhibitors from DNA preparations. It is used with
AquaGenomicTM
to purify PCR-ready DNA from fecal and soil materials, which
contain large amount of PCR inhibitors. Fecal
or soil material is incubated in AquaGenomic solution to extract the
DNA. The lysate is then mixed with AquaPrecipi solution and ethanol
to precipitate the DNA. The purified DNA is ready for PCR
amplification. AquaPrecipiTM is the most unique and
effective PCR inhibitor removal reagent on the market. While other
DNA stool kits produce approximately 10-60% of PCR success rate, our
AquaPrecipi purified DNA consistently gives 100% PCR success rate.
Fecal DNA analysis is a noninvasive method for monitoring wildlife
ecology, diagnosis of microbial infection, screening colorectal
cancers, and animal genotyping.
PCR amplification of human stool
DNA.
Lane 1: PCR of DNA precipitated by
isopropanol
Lane 2: PCR of DNA precipitated by
AquaPrecipi/ethanol.
Lane 3: PCR of DNA precipitated by
AquaPrecipi/isopropanol.
For detail extraction protocol, please click here. For ordering, please
click .
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