AquaPlasmid^TM 

AquaPlasmid is a novel aqueous solution-based technology for the isolation and purification of plasmid DNA. Its manufacturing cost is significantly lower than spin column based kits and the saving is passed on to the end users. AquaPlasmid protocol is as simple as standard alkaline lysis. It is scalable. Mini, midi, and maxiprep can be completed in as little as 30 minutes. The DNA yield can be up to 10 ug/ml culture. The isolated DNA is ultrapure and its A260/A280 ratio often achieves 2.0. AquaPlasmid offers significant cost, time, and labor savings to its end users.

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 AquaGenomic^TM 

AquaGenomic^TM is a nontoxic aqueous solution for genomic DNA isolation and purification from a variety of cells and tissues. It is a multi-functional solution. It can be used to suspend the cells, lyse the cells, extract the DNA, and precipitate other cellular components in a single step. You simply add AquaGenomic Solution to the cell pellet, vortex to extract the DNA, centrifuge to remove cell debris, and then add isopropanol to precipitate pure genomic DNA. Mini, midi, maxi, or even tissue prep can be completed in as little as 15 minutes. The yield is about 5-10 ug DNA from 1-2 million cultured human cells, 200 ul of whole blood, 15 mg of feces, 1 ml of microbial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues. 

 AquaRNA^TM 

AquaRNA^TM is a multifunctional aqueous solution for DNA, RNA, and protein extraction. It functions to lyse the cells, inactivate RNases, and extract DNA, RNA, and proteins. DNA and RNA are readily recovered from the lysate by isopropanol precipitation, while proteins remain in the supernatant and can be recovered by acetone precipitation. It allows you to simultaneously isolate DNA, RNA, and proteins for analyses from the same cells, tissue, or organism, making it possible to get more out of your limited and precious samples, or to save time, money and labor by doing three extractions concurrently. Additionally, AquaRNA^TM can be used to inactivate residual RNases in purified RNA and DNA samples to ensure their integrity and stability. 

AquaBluer^TM

AquaBluer^TM is a specially formulated 10x concentrated and Bluer redox indicator for assessing cell viability, cell proliferation, and cytotoxicity. The fluorescence intensity of AquaBluer^TM at 540ex/590em is proportional to the number of viable cells in the sample. In addition to being nontoxic, simple to use, sensitive, and reproducible, AquaBluer^TM has several distinctive advantages over comparable alamar blue and other similar reagents. First, it’s 10x concentrated and costs ~37% less. Secondly, it has 2x wider linear assay range and could accurately measure near confluent cultures where alamar blue fails. Finally, it’s more stable. It may be stored in the dark at 4-22 °C up to 12 months.

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AquaStool^TM

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 AquaPrecipi^TM 

AquaPrecipi^TM is a polymer solution for the removal of PCR inhibitors from DNA preparations. It is used with AquaGenomic^TM to purify PCR-ready DNA from fecal and soil materials, which contain large amount of PCR inhibitors. Fecal or soil material is incubated in AquaGenomic solution to extract the DNA. The lysate is then mixed with AquaPrecipi solution and ethanol to precipitate the DNA. The purified DNA is ready for PCR amplification. AquaPrecipi^TM is the most unique and effective PCR inhibitor removal reagent on the market. While other DNA stool kits produce approximately 10-60% of PCR success rate, our AquaPrecipi purified DNA consistently gives 100% PCR success rate. Fecal DNA analysis is a noninvasive method for monitoring wildlife ecology, diagnosis of microbial infection, screening colorectal cancers, and animal genotyping.

PCR amplification of human stool DNA.

Lane 1: PCR of DNA precipitated by isopropanol

      Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.

      Lane 3: PCR of DNA precipitated by AquaPrecipi/isopropanol.

 

 

 

 

 

 

 

 

 

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AquaPreserve^TM 

 

 

 

 

AquaPreserve^TM is the world’s first preservative with DNA, RNA, and protein extraction functionality. It may be used to streamline biospecimen collection, stabilization, transportation, storage, and extraction. It preserves biospecimens without causing biomolecule cross-linking, protects biospecimen integrity, simplifies biospecimen processing, and reduces processing heterogeneity. Freshly collected cells, tissues, or organs can be placed in AquaPreserve^TM solution to inactivate enzymatic activities without requiring snap freezing, shipped to the laboratory without using dry ice, and stored at room temperature or at 4 or –20 °C until biomolecule extraction. DNA, RNA, and proteins are extracted from AquaPreserve^TM preserved specimen directly by homogenization and sequential isopropanol and acetone precipitations without using additional DNA, RNA, and protein extraction kits.

 

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