AquaPlasmid^TM 

AquaPlasmid is a novel aqueous solution-based technology for the isolation and purification of plasmid DNA. Its manufacturing cost is significantly lower than spin column based kits and the saving is passed on to the end users. AquaPlasmid protocol is as simple as standard alkaline lysis. It is scalable. Mini, midi, and maxiprep can be completed in as little as 30 minutes. The DNA yield can be up to 10 ug/ml culture. The isolated DNA is ultrapure and its A260/A280 ratio often achieves 2.0. AquaPlasmid offers significant cost, time, and labor savings to its end users.

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 AquaGenomic^TM 

AquaGenomic^TM is a nontoxic aqueous solution for genomic DNA isolation and purification from a variety of cells and tissues. It is a multi-functional solution. It can be used to suspend the cells, lyse the cells, extract the DNA, and precipitate other cellular components in a single step. You simply add AquaGenomic Solution to the cell pellet, vortex to extract the DNA, centrifuge to remove cell debris, and then add isopropanol to precipitate pure genomic DNA. The yield is about 5-10 ug DNA from 1-2 million cultured human cells, 200-300 ul of whole blood, 15 mg of feces, 1 ml of microbial cultures, 5-10 mg of animal tissues, or 10-20 mg of plant tissues. 

 AquaRNA^TM 

AquaRNA^TM is a multifunctional aqueous solution for DNA, RNA, and protein extraction. It functions to lyse the cells, inactivate RNases, and extract DNA, RNA, and proteins. DNA and RNA are readily recovered from the lysate by isopropanol precipitation, while proteins are recovered by acetone precipitation. It allows you to simultaneously isolate DNA, RNA, and proteins for analyses from the same cells, tissue, or organism, making it possible to get more out of your limited and precious specimens. 

AquaBluer^TM

AquaBluer^TM is a specially formulated 10x concentrated and Bluer redox indicator for assessing cell viability, cell proliferation, and cytotoxicity. The fluorescence intensity of AquaBluer^TM at 540ex/590em is proportional to the number of viable cells in the sample. In addition to being nontoxic, simple to use, sensitive, and reproducible, AquaBluer^TM has several distinctive advantages over comparable alamar blue and other similar reagents. First, it’s 10x concentrated and costs ~37% less. Secondly, it has 2x wider linear assay range and could accurately measure near confluent cultures where alamar blue fails. Finally, it’s more stable. It may be stored in the dark at 4-22 °C up to 12 months.

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AquaStool^TM

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 AquaPrecipi^TM 

AquaPrecipi^TM is a polymer solution for the removal of PCR inhibitors from DNA preparations. It is used with AquaGenomic^TM to purify PCR-ready DNA from fecal and soil materials, which contain large amount of PCR inhibitors. Fecal or soil material is incubated in AquaGenomic solution to extract the DNA. The lysate is then mixed with AquaPrecipi solution and ethanol to precipitate the DNA. The purified DNA is ready for PCR amplification. AquaPrecipi^TM is the most unique and effective PCR inhibitor removal reagent on the market. While other DNA stool kits produce approximately 10-60% of PCR success rate, our AquaPrecipi purified DNA consistently gives 100% PCR success rate. Fecal DNA analysis is a noninvasive method for monitoring wildlife ecology, diagnosis of microbial infection, screening colorectal cancers, and animal genotyping.

PCR amplification of human stool DNA.

Lane 1: PCR of DNA precipitated by isopropanol

      Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.

      Lane 3: PCR of DNA precipitated by AquaPrecipi/isopropanol.

 

 

 

 

 

 

 

 

 

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AquaPreserve^TM 

 

 

 

 

AquaPreserve^TM is the world’s first preservative with DNA, RNA, and protein extraction functionality. It may be used to streamline blood biospecimen collection, stabilization, transport, storage, and DNA, RNA, and protein extraction. It protects biospecimen integrity, simplifies biospecimen processing, and reduces processing heterogeneity. Fresh or frozen whole blood may be stored in AquaPreserve^TM to inactivate enzymatic activities, DNA and RNA are recovered by isopropanol precipitation while proteins are recovered by acetone precipitation without using additional DNA, RNA, and protein extraction kits.

 

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ProMelt^TM

 

 

 

 

ProMelt^TM is a 1% SDS based protein solubilizing reagent. Proteins in cell lysate may be extracted with various detergents and subsequently precipitated by organic solvents such as acetone. However, it is well known that the protein pellet obtained by acetone precipitation is poorly soluble in aqueous solutions, making the protein yield and recovery highly variable. This poor solubility is due to the sequestering of the hydrophilic moieties in protein molecules after protein denaturation and precipitation. ProMelt is able to unfold the denatured proteins and makes their hydrophilic moieties become accessible to water molecule, therefore fully solubilizes the "insoluble" protein pellet. Please read the AquaRNA Total DNA/RNA/Protein Extraction Protocol for detail of its uses.

 

 

 

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ProSink^TM 

 

 

 

 

ProSink^TM is a protein precipitating reagent. Addition of ProSink to a solution containing proteins and nucleic acids will precipitate the proteins and leave the nucleic acids in the solution. The nucleic acids essentially free of proteins can subsequently be precipitated by isopropanol or ethanol. This ancillary reagent may be used to remove large amount of proteins and nucleases from cell lysate obtained by AquaPreserve and other cell lysis solutions to extract DNA and RNA from solid tissues and whole blood, which are rich in proteins and nucleases. Please read the AquaPreserve Blood Protocol for detail of its uses.