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Here you can find the latest
protocols of AquaPlasmidTM,
AquaGenomicTM,
AquaRNATM,
and AquaBluerTM.
You will notice how simple it is to use these products. We
continue to refine the protocols and expand the applications of our
products. Please let us know if you have any suggestions and
comments to our protocols and products.
AquaPlasmid
Protocols
AquaPlasmid protocol is similar to standard
alkaline lysis protocol. Both protocols consist of four simple
steps: (1) harvest the cells, (2) lyse the cells, (3) remove the
debris, and (4) pellet the plasmid DNA. However, the difference is
the purity of the isolated plasmid DNA. Alkaline lysis produces very
"dirty" plasmid DNA, which contains large amount of
protein and RNA. Toxic organic solvents, such phenol and chloroform,
are required to clean up the DNA before it can be used in subsequent
applications. AquaPlasmid isolated plasmid DNA is essentially free
of cellular impurities and the DNA is ready for down-stream
applications.
AquaPlasmid Miniprep
Protocol
For preparation of 5-10 ug of plasmid DNA from 1 ml overnight
bacterial culture.
1. Harvest the
Cells
Transfer 1 ml overnight culture to
a 1.5-ml microfuge tube. Centrifuge at ~15,000xg for 30 seconds at
room temperature (~22° C) to pellet the bacteria. Aspirate to
discard the supernatant as completely as
possible.
2. Lyse the
Cells
Add 50 ul deionized water to the bacterial pellet. Vortex
vigorously to fully resuspend the cells. Add 30
ul AquaLysis
Solution to the cell
suspension. Vortex vigorously for 10-20 seconds to mix the contents
well. Incubate at room temperature
for 3-4 minutes to lyse the cells.
3. Remove the
Debris
Add 30 ul AquaPlasmid Solution to the crude lysate. Touch-vortex (a few
seconds on and then a few seconds off) at top speed or shake
vigorously 10-15 times to mix the contents. Incubate at room
temperature for 3-4 minutes. Centrifuge at ~15,000xg for 5
minutes at room temperature to pellet the
debris.
4. Pellet the Plasmid DNA
Transfer
100 ul
clear lysate
to a clean 0.5-ml microfuge tube preloaded with 30
ul
of AquaPlasmid Solution.
Mix the contents by vortexing vigorously for 20-30 seconds.
Centrifuge at ~15,000xg for 5 minutes at room temperature to pellet
the plasmid DNA. Flip the tube to discard the supernatant. Fill the
tube with 70% ethanol by shooting the ethanol solution from a
squeeze bottle at the cap and sidewall of the tube, and then flip to
discard the ethanol. Repeat the 70% ethanol rinse 3 times. Flip the
tube forcefully a few times and blot it on a paper towel to remove
residual ethanol. Add 100 ul
deionized water and resuspend the DNA by vortexing for 20-30
seconds. Store the DNA solution at 4°
C or at –20°
C for long-term storage .
AquaPlasmid Midiprep
Protocol
For preparation of 50-100 ug of plasmid DNA from 10 ml bacterial
culture.
1. Harvest the
Cells
Transfer 10 ml overnight culture
to a 40-ml centrifuge tube. Centrifuge at ~15,000xg for 1 minute at
room temperature (~22° C) to pellet the bacteria. Aspirate or
pour off the supernatant as completely as
possible.
2. Lyse the
Cells
Add 500 ul deionized water to the bacterial pellet. Vortex
vigorously to fully resuspend the cells. Transfer the cell
suspension to a 2-ml microfuge tube. Add 300 ul AquaLysis Solution to the cell suspension. Vortex
vigorously for 10-20 seconds to mix the contents. Incubate at room
temperature
for 3-4 minutes to lyse the cells.
3. Remove the
Debris
Add 300 ul AquaPlasmid Solution to the crude lysate. Vortex vigorously
10-20 seconds to mix the contents and shake to break up the large
white aggregates. Incubate at room temperature for 3-4 minutes.
Centrifuge at ~15,000xg for 5 minutes at room temperature to pellet
the debris.
4. Pellet the Plasmid DNA
Transfer
1 ml clear lysate to a clean 2-ml microfuge tubes preloaded
with 300 ul
AquaPlasmid Solution.
Mix the contents by vortexing vigorously for 20-30 seconds.
Centrifuge at ~15,000xg for 5-10 minutes at room temperature to
pellet the plasmid DNA. Flip the tube to discard the supernatant.
Fill the tube with 70% ethanol by shooting the ethanol solution from
a squeeze bottle at the cap and sidewall of the tube, and then flip
to discard the ethanol. Repeat the 70% ethanol rinse 3 times. Flip
the tube forcefully a few times and blot it on a paper towel to
remove residual ethanol. Add 500 ul
deionized water and resuspend the DNA by vortexing for 30-60
seconds. Store the DNA solution at 4°
C or at –20°
C for long-term storage.
AquaPlasmid Maxiprep
Protocol
For preparation of 500-1000 ug of plasmid DNA from 100 ml bacterial
culture.
1. Harvest the
Cells
Transfer 100 ml overnight culture
to a 250-ml centrifuge bottle. Centrifuge at ~15,000xg for 2 minutes
at room temperature (~22° C) to pellet the bacteria. Aspirate or
pour off the supernatant as completely as
possible.
2. Lyse the
Cells
Add 5 ml deionized water to the
bacterial pellet. Vortex vigorously to fully resuspend the cells.
Add 3 ml AquaLysis Solution to the cell suspension. Shake to
mix the contents well and the vortex vigorously for 20-30 seconds.
Rotate the contents along the sidewall to wet the area that may
retain residual cell suspension. Incubate at room temperature
for 3-4 minutes to lyse the cells.
3. Remove the
Debris
Add 3 ml AquaPlasmid Solution to
the crude lysate. Gently shake and rotate the
contents along the sidewall to mix well. Vortex vigorously
for 20-30 seconds to break up the large white aggregates.
Incubate at room temperature for 3-4 minutes. Centrifuge at
~15,000xg for 5 minutes at room temperature to pellet the debris.
4. Pellet the Plasmid DNA
Transfer
10 ml clear lysate to a 40-ml centrifuge tube preloaded with
3 ml of AquaPlasmid Solution. Mix the contents by vortexing
vigorously for 20-30 seconds. Centrifuge at ~15,000xg for 10 minutes
at room temperature to pellet the plasmid DNA. Pour off the
supernatant. A white DNA pellet should be visible. Fill the tube
with 70% ethanol to about half full by shooting the ethanol solution
from a squeeze bottle along the sidewall of the tube, and then
rotate the ethanol solution to rinse the entire tube while slowly
pouring off the ethanol (Ensure that the DNA pellet remains attached
before pouring off the ethanol!). Repeat the 70% ethanol rinse 3
times. Tap the tube on a paper towel to remove residual ethanol. Add
1 ml deionized water to the DNA pellet and fully suspend the pellet
by pipetting the solution up and down. Transfer the DNA solution to
a 1.5-ml microfuge tube and centrifuge at ~15,000xg for 5 minutes to
pellet any insoluble. Transfer the clear DNA solution to another
clean tube. Store the DNA solution at 4° C or at –20° C for
long-term
storage .
AquaPlasmid 96-Well Plate Protocol
This protocol can be used to prepare
miniprep DNA in 96-well high-throughput format. It yields 5-10
ug of plasmid DNA per
well.
1. Harvest the
Cells
Grow the bacteria in 0.5-1 ml
overnight culture in 96-well deep-well (1-2 ml) plate in a
37° C shaker overnight. The plate should be
sealed with a microporus tape. Centrifuge the overnight bacterial
culture in bucket rotors that hold deep-well plates at allowed
maximal speed (>1,000xg) for 15 minutes at room temperature
(~22° C) to pellet the bacteria. Aspirate to
remove the medium. If only rotors for standard microtiter plates are
available, standard 48-well plates can be used to grow the bacteria
in 0.5 ml culture medium.
2. Lyse the
Cells
Add 50 ul deionized water to each well. Shake the plate by hands
or on a plate shaker at top speed to fully resuspend the cells. Add
30 ul AquaLysis
Solution to each well.
Shake the plate by hands or on the shaker at top speed for 10-20
seconds to mix the contents. Incubate at room temperature
for 3-4 minutes to lyse the cells.
3. Remove the
Debris
Add 30 ul AquaPlasmid Solution to each well. Shake the plate by hands
or on the shaker for 20-30 seconds to mix the contents. Incubate at
room temperature for ~5 minutes. Centrifuge the plate at allowed
maximal speed for 15 minutes at room temperature to pellet the
debris.
4. Pellet the Plasmid DNA
Carefully
transfer 100 ul
clear lysate
to corresponding well in a clean plate. Add 30
ul
AquaPlasmid Solution
to each well. Shake the plate by hands or on a plate shaker at top
speed for 1-2 minutes to mix the contents. Centrifuge the plate at
allowed maximal speed for 15 minutes at room temperature to pellet
the plasmid DNA. Flip the plate to discard the supernatant. Fill the
wells with 70% ethanol to ~80% full by gently shooting the ethanol
solution from a squeeze bottle at the sidewall of the wells, and
then flip the plate to discard the ethanol. Repeat the 70% ethanol
rinse 3 times. Flip the plate forcefully a few times and blot it on
a paper towel to remove residual ethanol. Add 100 ul
deionized water to each well. Shake the plate by hands or on a plate
shaker at top speed for 1-2 minutes to resuspend the DNA. Store the
DNA solution at 4°
C or at –20°
C for long-term storage .
AquaGenomic
Protocols
The uniqueness of AquaGenomicTM
is its ability to perform multiple functions in a single step:
suspending the cells, lysing the cells, extracting the DNA, and
precipitating the cell debris. The AquaGenomic protocols consist
of four simple steps: (1) harvest the cells, (2) extract the
DNA, (3) pellet the debris, and (4) pellet the DNA.
AquaGenomic Cell
Protocol
This protocol can be used to prepare 5-10
ug of genomic DNA from 1-2
million cultured cells. For
other preparation scales, use 100 ul AquaGenomic Solution for each million
nucleated cells. AquaGenomic Solution contains detergents. Personal
protections, such as rubber gloves, chemical safety goggles, and
laboratory coat, should be worn when handling AquaGenomic
Solution.
1. Harvest the
Cells
For cultured cells: Pellet ~0.5-2 million
cultured cells in a 1.5-ml microfuge tube by centrifugation at
2,000xg for 2 minutes. Aspirate or decant to discard the supernatant
and leave behind the cell pellet.
2. Extract the
DNA
Add
100 ul
of AquaGenomic Solution to the cell pellet. Suspend and lyse the
cells by vortex vigorously for 30-60 seconds. Incubate at room
temperature (or at 60°
C for better yield) for 4 minutes.
3. Pellet the
Debris
Vortex
vigorously for 30-60 seconds and centrifuge the sample at
10,000-20,000xg for 2 minutes to pellet the
debris.
4. Pellet the DNA
Transfer the supernatant (~90
ul) to a fresh 1.5-ml
microfuge tube. Add 0.7 volume (~60 ul, do not use more than 1 volume or
it may become cloudy) of 100% isopropanol and mix the
contents by vortexing for 30 seconds. Centrifuge at 10,000-20,000xg
for 2 minutes to pellet the DNA. Flip to discard the
supernatant. Fill the microfuge tube with 70% ethanol by shooting
the ethanol solution from a squeeze bottle at the cap of the tube,
and then flip to discard the ethanol. Repeat the 70% ethanol
rinse 2 times. Flip
the tube and tap it several times on a paper towel to remove
residual ethanol (Do not over dry the DNA pellet or it may be
difficult to re-hydrate). Add 50-100 ul
of TE buffer or deionized water, incubate
at room temperature 15-30 min to fully rehydrate the
pellet,
pipette up-and-down or vortex vigorously to suspend the DNA.
Store
the DNA solution at 4°
or at –20°
C for long-term storage.
AquaGenomic Blood Protocol
(We recommend AquaRNA Blood
Protocol for blood and bone marrow DNA extraction.
AquaRNA significantly reduces hemoglobin and
heme contamination of blood DNA due to its potent protein
extraction and removal ability.)
This
protocol can be used to prepare ~5-6 ug
of genomic DNA from 100 ul
of whole blood (fresh or frozen). It requires a RBC Lysis Solution
(Product # 4015, not included) to lyse the red blood cells prior to
DNA extraction. AquaGenomic Solution contains detergents. Personal
protections, such as rubber gloves, chemical safety goggles, and
laboratory coat, should be worn when handling AquaGenomic
Solution.
1. Harvest the Cells
Add
50 ul
of RBC Lysis Solution to 100 ul
whole blood. Mix the contents by touch-vortex (a few seconds on and
a split second off) for 20-30 times at top speed to lyse the red
blood cells. Add 1 ml deionized water and vortex to wash the cells.
Centrifuge at 10,000-20,000xg for 1 minute to pellet the white blood
cells and nuclei. Aspirate to remove the supernatant. Add 1 ml
deionized water to the cell pellet, vortex to wash the pellet, and
centrifuge to pellet the cells. Aspirate to remove the
supernatant.
2. Extract the
DNA
Add
100 ul
of AquaGenomic Solution to the cell pellet. Suspend and lyse the
cells by pipetting and vortex. Incubate at room temperature (or at
60° C for better yield) for 4 minutes.
3. Pellet the
Debris
Vortex
vigorously for 30-60 seconds and centrifuge at 10,000-20,000xg for 2
minutes to pellet the debris.
4. Pellet the DNA
Transfer
the supernatant (~100 ul)
to a fresh 0.5-ml microfuge tube. Add 0.7 volume (~70
ul,
do not use more than 1 volume or
it may become cloudy) of 100% isopropanol and mix the
contents by vortexing for 30 seconds. Centrifuge at 10,000-20,000xg
for 2 minutes to pellet the DNA. Decant to discard the supernatant.
Fill the microfuge tube with 70% ethanol by shooting the ethanol
solution from a squeeze bottle at the cap of the tube, and then
decant to discard the ethanol. Repeat the 70% ethanol rinse 2 times.
Flip the tube and tap it several times on a paper towel to remove
residual ethanol (Do not over dry the DNA pellet or it may be
difficult to re-hydrate). Add 50 ul
of TE buffer or deionized water, incubate
at room temperature 15-30 min to fully rehydrate the
pellet,
pipette up-and-down or vortex vigorously to suspend the DNA.
Store the DNA solution at 4° or at –20° C for long-term
storage .
AquaGenomic 96-Well Plate Blood
Protocol
This protocol requires the use of a
bucket rotor that hold 96-well plates (1 or 2-ml deep-well plates)
with a relative centrifugation force >3,000xg, such as Beckman
S5700. Make sure your plates can sustain the required RCF.
AquaGenomic Solution contains detergents. Personal protections, such
as rubber gloves, chemical safety goggles, and laboratory coat,
should be worn when handling AquaGenomic
Solution.
1. Harvest the
Cells
Load 100 ul whole blood into each of the
96 wells. Add 50 ul
of RBC Lysis Solution (Product # 4015) to each well. Seal
the plate with an adhesive cover. Shake the plate on a plate shaker
at top speed for one minute to lyse the red blood cells (RBC).
Add 1 ml deionized water to each well and shake the plate for one
minute to wash the cells. Centrifuge the plate at allowed maximal
speed (>3,000xg) for 5 minutes to pellet the white blood
cells and nuclei. Aspirate to remove the red supernatant. Add 1 ml
deionized water to the pellet, shake the plate for a minute to wash
the pellet, centrifuge for 5 minutes to pellet the cells, and
aspirate to remove the supernatant.
2. Extract the
DNA
Add
100 ul
of AquaGenomic Solution to each well. Pipette up-and-down and shake
the plate vigorously for 2 minutes to suspend and lyse the cells.
Incubate the plate at room temperature for 5
minutes.
3. Pellet the
Debris
Centrifuge the plate at allowed maximal
speed for 15 minutes at room temperature to pellet the cell debris.
4. Pellet the DNA
Transfer the supernatant (~100
ul) to corresponding well in a fresh
plate. Add 0.7 volume (~70 ul,
do not use more than 1 volumeor
it may become cloudy) of 100% isopropanol and mix the
contents by shaking the plate for 2 minutes on a plate shaker (do
not mix the contents by pipetting or the DNA may stick to the
pipette tips). Centrifuge at allowed maximal speed for 15 minutes to
pellet the DNA. Flip or aspirate to remove the supernatant. Add 1 ml
70% ethanol to each well, and then flip or aspirate to remove the
ethanol. Repeat the 70% ethanol rinse 2 times. Centrifugation prior
to ethanol removal may be required if the DNA pellets become loss.
Invert the plate and tap it on a paper towel to drain off residual
ethanol (Do not over dry the DNA pellet or it may be difficult to
re-hydrate). Add 50 ul
of deionized water or TE buffer to each well, and incubate
at room temperature 15-30 min to fully rehydrate the pellet.
Pipette or shake the plate for 5 minutes to suspend the DNA. Store
the DNA solution at 4° or
at –20° C
for long-term storage.
AquaGenomic Swab
Protocol
This protocol can be used to
prepare 5-10 ug of
genomic DNA from one buccal swab. It is a simple, fast, and
non-invasive method to obtain genomic DNA from individuals for
epidemiological, pharmacogenomic, genealogical,
paternal, and forensic studies. AquaGenomic Solution contains
detergents. Personal protections, such as rubber gloves, chemical
safety goggles, and laboratory coat, should be worn when handling
AquaGenomic Solution.
1. Harvest the
Cells
Swirl
and rub your tongue against the inside of your cheek and gum for ~5
times. Subsequently place a sterile cotton tipped swab into your
mouth. Move the swab around the mouth 10-20 times to rub different
areas along the cheek-gum juncture and soak up the saliva. Swabs may
also be used to collect cells from animals, tissue cultures, and
forensic items to obtain genomic
DNA.
2. Extract the
DNA
For
fresh wet swab: Immediately place the wet swab into a 1.5-ml
microfuge tube preloaded with 200 ul
of AquaGenomic Solution. For dry swab: Soak the dry swab in 400
ul
of 50% diluted AquaGenomic Solution (200 ul
AquaGenomic Solution and 200 ul
deionized water) in a 1.5-ml microfuge tube for 4 minutes. Loosely
rotate the swab back-and-forth as well as pump it up-and-down 10-20
times in the solution. Squeeze off as much as possible the liquid
from the cotton tip against the wall of the tube. Discard the swab.
Incubate at room temperature (or at 60°
C for better yield) for 4 minutes.
3. Pellet the
Debris
Vortex
the sample vigorously for 30-60 seconds and centrifuge at
10,000-20,000xg for 2 minutes to pellet the
debris.
4. Pellet the DNA
Transfer
the clear supernatant (~180 ul)
to a fresh 1.5-ml microfuge tube. Add 0.7 volume (~120
ul,
do not use more than 1 volume or
it may become cloudy) of 100% isopropanol and mix the
contents by vortexing for 30 seconds. Centrifuge at 10,000-20,000xg
for 2 minutes to pellet the DNA. Flip to discard the
supernatant. Fill the microfuge tube with 70% ethanol by shooting
the ethanol solution from a squeeze bottle at the cap of the tube,
and then flip to discard the ethanol. Repeat the 70% ethanol
rinse 2 times. Flip
the tube and tap it several times on a paper towel to remove
residual ethanol (Do not over dry the DNA pellet or it may be
difficult to re-hydrate). Add 50-100 ul
of TE buffer or deionized water, incubate
at room temperature 15-30 min to fully rehydrate the
pellet,
pipette up-and-down or vortex vigorously to suspend the DNA.
Store
the DNA solution at 4°
or at –20°
C for long-term storage.
AquaGenomic Saliva Protocol
Saliva is one of the most accessible
sources of genomic DNA from human subjects. About 10-20
ug of genomic DNA can be
readily extracted from 1 ml mouthwash using 200 ul of AquaGenomic Solution. AquaGenomic
Solution contains detergents. Personal protections, such as rubber
gloves, chemical safety goggles, and laboratory coat, should be worn
when handling AquaGenomic Solution.
1. Harvest the
Cells
Swirl and rub your tongue against the
inside of your cheek and gum for ~5 times. Spit the saliva into a
50-ml conical tube. Take (do not swallow) 10 ml (one tablespoon) of
Scope Mouthwash and swish it vigorously around the inside of your
mouth for 20 times. Carefully spit the mouthwash into the tube.
Vortex to mix the contents well. Transfer 1 ml mouthwash to a 1.5-ml
microfuge tube and centrifuge at 10,000-20,000xg for 1 minute to
pellet the cells. Aspirate to remove the
supernatant.
2. Extract the
DNA
Add 200 ul of AquaGenomic Solution to the cell
pellet. Pipette up and down to mix the contents. Vortex the tube
vigorously for 30-60 seconds. Incubate at room temperature (or at
60° C for better yield) for 4
minutes.
3. Pellet the
Debris
Vortex the tube vigorously for 30 seconds
and centrifuge the sample at 10,000-20,000xg for 4 minutes to pellet
the debris.
4. Pellet the DNA
Transfer the supernatant (~180
ul) to a fresh 1.5-ml
microfuge tube. Add 0.7 volume (~120 ul, do not use more than 1 volume or
it may become cloudy) of 100% isopropanol and mix the
contents by vortexing for 30 seconds. Centrifuge at 10,000-20,000xg
for 2 minutes to pellet the DNA. Flip to discard the
supernatant. Fill the microfuge tube with 70% ethanol by shooting
the ethanol solution from a squeeze bottle at the cap of the tube,
and then flip to discard the ethanol. Repeat the 70% ethanol
rinse 2 times. Flip
the tube and tap it several times on a paper towel to remove
residual ethanol (Do not over dry the DNA pellet or it may be
difficult to re-hydrate). Add 100 ul
of TE buffer or deionized water, incubate
at room temperature 15-30 min to fully rehydrate the
pellet,
pipette up-and-down or vortex vigorously to suspend the DNA.
Store the DNA
solution at 4° or at –20° C for long-term storage.
AquaGenomic Tissue Protocol
AquaGenomic does not require the usual
4-16 hours of proteinase K digestion to extract genomic DNA from
solid tissues. DNA can be isolated from tissues in as little as 15
minutes. About 10-20 ug of
genomic DNA can be isolated from 10 mg of tissues. AquaGenomic
Solution contains detergents. Personal protections, such as rubber
gloves, chemical safety goggles, and laboratory coat, should be worn
when handling AquaGenomic Solution.
1. Harvest the
Cells
Dissect
the tissues and stored at –20°
to -70° C until process. Cut out a ~2 mm cube (~10 mg) of frozen
tissue (liver, lung, kidney, heart, brain, spleen, muscle, tail,
etc.). Place the tissue in 200 ul
of AquaGenomic Solution in a pestle-and-tube homogenizer or a 1.5-ml
microfuge tube containing ~50 ul
1-3 mm glass or ceramic beads.
2. Extract the
DNA
By homogenization: Homogenize the tissue
at room temperature. After homogenization, add 1/10 volume (~25
ul) of 100% isopropanol to
the sample to reduce the forming. Briefly vortex and immediately
pour the sample into a 1.5-ml microfuge tube. Incubate at room
temperature (or at 60° C for better yield) for 4 minutes.
By
60°
C incubation: Incubate the tissue with the beads in AquaGenomic
Solution at 60° C for 4 hours. At the end of incubation, vortex and
flick the tube vigorously to disrupt the tissue. If beads are not
available, the tissue can be disrupted by a pestle or pipette
tip .
3. Pellet the
debris
Vortex
and flick the sample vigorously for 30-60 seconds and centrifuge at
10,000-20,000xg for 4 minutes to pellet the
debris.
.
4. Pellet the DNA
Transfer
the clear supernatant (~120 ul)
to a fresh 1.5-ml microfuge tube. Add 0.7 volume (~80
ul,
do not use more than 1 volume or
it may become cloudy) of 100% isopropanol and mix the
contents by vortexing for 30 seconds. Centrifuge at 10,000-20,000xg
for 2 minutes to pellet the DNA. Flip to discard the supernatant.
Fill the microfuge tube with 70% ethanol by shooting the ethanol
solution from a squeeze bottle at the cap of the tube, and then flip
to discard the ethanol. Repeat the 70% ethanol rinse 2 times. Flip
the tube and tap it several times on a paper towel to remove
residual ethanol (Do not over dry the DNA pellet or it may be
difficult to re-hydrate). Add 100 ul
of TE buffer or deionized water, incubate
at room temperature 15-30 min to fully rehydrate the
pellet,
pipette up-and-down or vortex vigorously to suspend the DNA.
Store
the DNA solution at 4°
or at –20°
C for long-term storage.
AquaGenomic Plant Protocol
This
protocol may be used to isolate ~10-20 ug
of genomic DNA from 25 mg of plant tissues, using 250 ul
AquaGenomic Solution. AquaGenomic Solution contains detergents.
Personal protections, such as rubber gloves, chemical safety
goggles, and laboratory coat, should be worn when handling
AquaGenomic Solution.
1. Harvest the Cells
Weigh
out ~25 mg of fresh or frozen plant tissue. Cut the tissue into
small pieces. Place them in 250 ul
of AquaGenomic Solution in a pestle-and-tube
homogenizer.
2. Extract the
DNA
Homogenize
the tissue at room temperature. After homogenization, add 1/10
volume (~30 ul)
of 100% isopropanol to the sample to reduce forming. Briefly vortex
and immediately pour the sample into a 1.5-ml microfuge tube.
Incubate at 60°
C for 10 minutes.
3. Pellet the
debris
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